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ProteomicsMEF2A, MEF2C, and MEF2D Interactomes in Mouse MA-10 Leydig Cells
In steroidogenic Leydig cells, MEF2A, MEF2C, and MEF2D are key regulators of genes involved in steroid hormone synthesis, reproductive function, and oxidative stress defense. Here we used TurboID proximity-mediated biotinylation to map the protein-protein interaction networks of MEF2A, MEF2C, and ME...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2024-11-15 | MSV000096432 | MassIVE
TranscriptomicsDetermining the Program of Leydig Cell Development
In the present study, microarray analysis was performed on RNA isolated from purified SLCs, PLCs, ILCs, ALCs and bone stem cells, using Affymetrix Rat Genome RAE230 2.0 arrays which monitor ~30,000 transcripts from over ~28,000 well-substantiated genes. The focus is on the differences and similarit...
ORGANISM(S): Rattus norvegicus 
2011-12-28 | E-GEOD-26703 | biostudies-arrayexpress
ProteomicsComprehensive and quantitative analysis of the changes in proteomic and phosphoproteomic profiles during stimulation and repression of steroidogenesis in MA-10 Leydig cells.
In the present study, we have used a quantitative LC-MS/MS approach using total and phosphopeptide-enriched proteins to identify the changes that occur in the proteome and phosphoproteome of MA-10 Leydig cells during both the stimulatory phase (Fsk/cAMP treatment) and inhibitory phase (AICAR-mediate...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-10-17 | MSV000090539 | MassIVE
TranscriptomicsPurification and transcriptomic analysis of mouse fetal Leydig cells reveals candidate genes for disorders of sex development
To examine the transcriptome of early testicular somatic cells during gonadogenesis at 12.5dpc RNA sequencing (RNA-Seq) was performed on murine primary testicular cell lineages isolated from the Sf1-eGFP line by FACS. The three main somatic cell lineages of the testis were isolated: the Sertoli cell...
ORGANISM(S): Mus musculus 
2015-05-21 | E-GEOD-65498 | biostudies-arrayexpress
ProteomicsLabel-free based quantitative proteomics analysis of primary neonatal porcine Leydig cells exposed to the persistent contaminant 3-methylsulfonyl-DDE
Evidence that persistent environmental pollutants may target the male reproductive system is increasing. The male reproductive system is regulated by secretion of testosterone by testicular Leydig cells, and perturbation of Leydig cells function may have ultimate consequences. 3-methylsulfonyl-DDE (...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2016-01-05 | PXD003165 | Pride
Endocrine disruption Proteomics 3-methylsulfonyl-dde Ipa Leydig cells Sus scrofa Persistent organic pollutants Steroidogenesis 
TranscriptomicsEffect of AMPK activation by AICAR on MA-10 Leydig cell transcriptome
Steroid hormones regulate essential physiological processes and inadequate levels are associated with various pathological conditions. In testosterone-producing Leydig cells, steroidogenesis is strongly stimulated by LH via its receptor leading to increased cAMP production and expression of the ster...
ORGANISM(S): Mus musculus 
2014-08-19 | E-GEOD-50118 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse Leydig cells and Sertoli cell rich areas (SCRA) exposed to DEHP
This SuperSeries is composed of the following subset Series:; GSE13237: Effect of DEHP on adult mouse Sertoli cells rich areas (SCRA); GSE13240: Effect of DEHP on adult mouse Leydig cells Experiment Overall Design: Refer to individual Series
ORGANISM(S): Mus musculus 
2009-02-27 | E-GEOD-13241 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of adult mouse Leydig cells exposed to DEHP
BACKGROUND: Phthalates are manmade industrial additives used mostly as plasticizers. In addition to their deleterious effects on male genital development, population studies have recently documented correlations between phthalates exposure and subtle impacts on reproductive tract development and on ...
ORGANISM(S): Mus musculus 
2009-04-10 | E-GEOD-13240 | biostudies-arrayexpress
TranscriptomicsGenome-wide analysis of gene expression in mLTC-1 cells following siRNA-mediated Gata4 inhibition
The effects of Gata4 inhibition on global gene expression in mLTC-1 cells was analysed. Using microarray analysis we identified a set of genes that are downregulated or upregulated following siRNA-mediated silencing of Gata4 in the murine Leydig tumor cell line mLTC-1. Among the downregulated genes...
ORGANISM(S): Mus musculus 
2015-02-23 | E-GEOD-63320 | biostudies-arrayexpress
TranscriptomicsRiboTag analysis of actively translated mRNAs in Sertoli and Leydig cells in vivo
Analysis of Sertoli and Leydig cell “translatome” utilizing an in vivo ribosome tagging strategy (RiboTag) that allows a detailed and physiologically relevant characterization of the polysome-associated mRNAs in vivo. Although progress has been made in the identification of specific transcripts th...
ORGANISM(S): Mus musculus 
2013-04-06 | E-GEOD-45799 | biostudies-arrayexpress
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