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ProteomicsComparison of LFQ and TMT-based quantitative proteomics towards toxicology research
Here, we aimed to compare two of the most commonly used global proteomics strategies, label-free quantification (LFQ) and tandem mass tag-based quantification (TMT) to identify the most suitable tool for toxicology research questions on the systemic level. Using a biological case study of HepG2 expo...
ORGANISM(S): Homo sapiens (Human) 
2020-12-23 | PXD020248 | Pride
Toxicoproteomics Benzo[a]pyrene Tandem mass tag (tmt) Label free-quantification (lfq) Hepg2 
ProteomicsComparison of DIA and TMT based protein quantification in complex background
Label free quantification (LFQ) and isobaric labelling quantification (ILQ) are among the most popular protein quantification workflows in discovery proteomics. Here, we compared the TMT 10-plex workflow to label free single shot data-independent acquisition (DIA) method on a controlled sample set. ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2020-01-29 | MSV000084859 | MassIVE
ProteomicsBenchmarking LFQ, SILAC and MS2/MS3-based TMT quantification strategies for large-scale phosphoproteomics
Comprehensive mass spectrometry (MS)-based proteomics is now feasible, but reproducible and multiplexed quantification remains challenging especially for analysis of post-translational modifications (PTMs), such as phosphorylation. Here we compared the most popular quantification techniques for phos...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-03-13 | PXD007145 | Pride
Phosphoproteomics Ms3 Ms2 Tmt Silac Lfq Technical Benchmark Quantification 
UnknownProteomics in Practice: A Case Study Highlighting Tandem Mass Tag-Based MS for Quantitative Profiling of Extracellular Vesicles and Application to Irradiated Fibroblasts.
Not available
S-EPMC12934204 | biostudies-literature
ProteomicsComparison of LFQ and IPTL for protein identification and relative quantification
Mass spectrometry-based quantitative proteome profiling is most commonly performed by label-free quantification (LFQ), stable isotopic labeling with amino acids in cell culture (SILAC), and reporter-ion based isobaric labeling methods (TMT and iTRAQ). Isobaric peptide termini labeling (IPTL) was des...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026942 | Pride
Tmt Silac Lfq Quantitative proteomics Iptl 
UnknownA Bioconductor workflow for processing, evaluating, and interpreting expression proteomics data.
Not available
S-EPMC10683783 | biostudies-literature
UnknownComparative Proteome Profiling of Saliva Between Estrus and Non-Estrus Stages by Employing Label-Free Quantitation (LFQ) and Tandem Mass Tag (TMT)-LC-MS/MS Analysis: An Approach for Estrus Biomarker Identification in Bubalus bubalis.
Not available
S-EPMC9217162 | biostudies-literature
UnknownBenchmarking common quantification strategies for large-scale phosphoproteomics.
Not available
S-EPMC5849679 | biostudies-literature
ProteomicsProteome-wide evaluation of two common protein quantification methods
Proteomics experiments commonly aim to estimate and detect differential abundance across all expressed proteins. Within this experimental design, some of the most challenging measurements are small fold changes for lower abundance proteins. While bottom-up proteomics methods are approaching comprehe...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-04-05 | PXD007683 | Pride
Tmt Lfq Maxquant 
ProteomicsProteome-wide evaluation of two common protein quantification methods
Proteomics experiments commonly aim to estimate and detect differential abundance across all expressed proteins. Within this experimental design, some of the most challenging measurements are small fold changes for lower abundance proteins. While bottom-up proteomics methods are approaching comprehe...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932) 
2019-02-15 | MSV000083453 | MassIVE
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