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ProteomicsKOPI - Kinase inhibitor proteome impact analysis
Kinase inhibitors often exhibit on/off-target effects, and efficient data analysis is essential for assessing these effects on the proteome. We developed a workflow for rapidly performing such a proteomic assessment, termed as kinase inhibitor proteome impact analysis (KOPI). We demonstrate KOPI’s ...
ORGANISM(S): Homo Sapiens (human) 
2022-06-16 | PXD029924 | JPOST Repository
ProteomicsPhosphosites of the yeast centrosome component Spc110 contribute to mitotic exit
Spc110 is an essential component of the spindle pole body (SPB), the yeast equivalent of the centrosome, that recruits the gamma-tubulin complex to the nuclear side of SPB to form the mitotic spindle. Here we identify two phosphosites (S11 and S36) in old (from maternal origin) Spc110. Yeast expres...
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) 
2023-04-03 | PXD033085 | JPOST Repository
ProteomicsK562 Elutriation cell cycle phase fractions 2D-MS-CETSA
K562 cells were synchronized by cell size difference based Elutriation scheme into G1, S and G2 fractions. Three biological replicates were collected on different days, and assembled into a 2D-CETSA scheme with 6 different heating temperatures (37ºC, 47ºC, 50ºC, 52ºC,54ºC and 57ºC), (Figure 1A, Meth...
ORGANISM(S): Homo Sapiens (human) 
2018-05-08 | PXD009171 | JPOST Repository
ProteomicsK562 Chemical arrest cell cycle phase fractions 2D-MS-CETSA
K562 cells were synchronized by double thymidine block(DTB) based chemical arrest scheme into G1/S, S and Prometaphase(PM) fractions. Three biological replicates were collected on different days, and assembled into a 2D-CETSA scheme with 6 different heating temperatures (37ºC, 47ºC, 50ºC, 52ºC,54ºC ...
ORGANISM(S): Homo Sapiens (human) 
2018-05-08 | PXD009146 | JPOST Repository
ProteomicsComplete sequences of the velvet worm slime proteins reveal that slime formation is enabled by disulfide bonds and intrinsically disordered regions
The slime of velvet worms (Onychophora) is a strong and fully biodegradable protein material, which upon ejection undergoes a fast liquid-to-solid transition to ensnare prey. However, the molecular mechanisms of slime self-assembly are still not well understood, notably because the primary structure...
ORGANISM(S): Cellular Organisms 
2022-09-20 | PXD031722 | JPOST Repository
ProteomicsCrosslinking MS of Antibody-HLA-A*11:01
Cross-linking mass spectrometry data of cross-linked antibody to human leukocyte antigen (HLA-A*11:01). For antibodies 2E3, 1E3 and 3H10.
ORGANISM(S): Homo Sapiens (human) 
2023-11-23 | PXD038059 | JPOST Repository
ProteomicsA TCR blocking HLA-A*11:01-specific human monoclonal antibody
Cross-linking mass spectrometry and Targeted cross-linking mass spectrometry for a TCR blocking human monoclonal antibody targeting HLA-A*11:01
ORGANISM(S): Homo Sapiens (human) 
2025-06-02 | PXD053836 | JPOST Repository
ProteomicsThe human pathobiont Malassezia furfur secreted protease MfSAP1 regulates cell dispersal and exacerbates skin inflammation
Malassezia forms the dominant eukaryotic microbial community on the human skin. The Malassezia genus possesses a repertoire of secretory hydrolytic enzymes involved in protein and lipid metabolism which alter the external cutaneous environment. The exact role of most Malassezia secreted enzymes, inc...
ORGANISM(S): Cellular Organisms 
2022-11-11 | PXD033336 | JPOST Repository
ProteomicsG9a/GLP inhibition during ex vivo lymphocyte expansion increases in vivo cytotoxicity of engineered TCR-T cells against hepatocellular carcinoma
Engineered T cells transiently expressing tumor-targeting receptors are an attractive form of engineered T cell therapy as they carry no risk of insertional mutagenesis or long-term adverse side-effects. However, multiple rounds of treatment are often required, increasing patient discomfort and cost...
ORGANISM(S): Homo Sapiens (human) 
2023-01-19 | PXD039542 | JPOST Repository
ProteomicsDomain-specific p53 mutants activate EGFR by distinct mechanisms exposing tissue-independent therapeutic vulnerabilities
Mis-sense mutations affecting TP53 promote carcinogenesis both by inactivating its tumor suppressive functions, and by conferring aberrant pro-carcinogenic activities. We report here that mis-sense mutants in the p53 DNA-binding domain (DBD) and the transactivation domain (TAD) unexpectedly activate...
ORGANISM(S): Homo Sapiens (human) 
2023-02-17 | PXD031725 | JPOST Repository
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