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MetabolomicsAn NMR metabolomics analysis pipeline for human neutrophil samples with limited source material
Untargeted 1H NMR metabolomics is a robust and reproducible approach to study metabolism in biological samples, providing unprecedented insight into altered cellular processes associated with human diseases. Metabolomics is increasingly used alongside other -omics techniques, such as proteomics and ...
2025-11-06 | MTBLS6220 | MetaboLights
MetabolomicsSpecific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast
Glucose as a source of energy is centrally important to our understanding of life. We investigated the cell division-quiescence behavior of the fission yeast Schizosaccharomyces pombe under a wide range of glucose concentrations (0-111 mM). The mode of S. pombe cell division under a microfluidic per...
2017-07-06 | MTBLS316 | MetaboLights
MetabolomicsLimited oxygen availability in standard cell culture alters metabolism and function in terminally differentiated cells

Cell culture is generally considered to be hyperoxic. However, the importance of cellular oxygen consumption is often underappreciated, with rates of oxygen consumption often sufficient to cause hypoxia at cell monolayers. We initially focused on cultured adipocytes as a terminally differentiated...

2024-02-21 | MTBLS6677 | MetaboLights
MetabolomicsLimited nutrient availability in the tumor microenvironment renders pancreatic tumors sensitive to allosteric IDH1 inhibitors

Nutrient-deprived conditions in the tumor microenvironment (TME) restrain cancer cell viability due to increased free radicals and reduced energy production. In pancreatic cancer cells, a cytosolic metabolic enzyme, wild-type isocitrate dehydrogenase 1 (wtIDH1), enables the adaptation to these co...

2022-04-29 | MTBLS4722 | MetaboLights
MetabolomicsLimited vertical transmission but profound immune dysregulation at the maternal-fetal interface after SARS-CoV-2 infection

The endemic of SARS-CoV-2 underscores the imperative to elucidate its effects on pregnancy, particularly regarding the potential for vertical transmission and its influence on the early maternal-fetal interface, which remain insufficiently defined. To address this, we performed an extensive cohor...

2026-03-05 | MTBLS13103 | MetaboLights
MetabolomicsLimited survival and impaired hepatic fasting metabolism of glucose, amino acids and lipids in mice with constitutive Rag GTPase signaling
The mechanistic target of rapamycin complex 1 (mTORC1) integrates cellular nutrient signaling and hormonal cues to control metabolism. We have previously shown that constitutive nutrient signaling to mTORC1 by means of genetic activation of RagA (RagAGTP) in mice resulted in a fatal energetic crisis...
2021-05-11 | MTBLS2397 | MetaboLights
ProteomicsSimple and efficient micro-SPE tip-based sample preparation workflow to enable sensitive proteomic profiling of limited samples
In-depth LC-MS-based proteomic profiling of limited samples has been problematic due, in large part, to the inefficiency of sample preparation and attendant sample losses. To address this issue, we developed On-Micro Solid-phase Extraction Tip-based (OmSET) sample preparation for limited biological...
ORGANISM(S): Homo sapiens (Human) 
2021-02-23 | PXD022313 | Pride
Micro-spe tip Omset Limited sample On-micro solid-phase extraction tip Sample preparation Stagetip 
ProteomicsThe $10 proteome: low-cost, deep and quantitative proteome profiling of limited sample amounts using the Orbitrap Astral and timsTOF Ultra 2 mass spectrometers
Mass spectrometry (MS)-based proteomics remains technically demanding and prohibitively expensive for many large-scale or routine applications, with per-sample costs of hundreds of dollars or more. To democratize access to proteomics and facilitate its integration into more high-throughput multiomic...
ORGANISM(S): Homo sapiens (Human) 
2026-02-04 | PXD066701 | Pride
Limited sample input $10 proteome 
TranscriptomicsTranscription profiling of human skin bioposis from patients with diffuse scleroderma and limited scleroderma
RNA expression profiling was carried out for systemic sclerosis (scleroderma) on skin biopsies obtained from both diffuse scleroderma (dcSSc) and limited scleroderma (lcSSc) subjects patients and compared to normal subjects. The patient group comprised of 4 dcSSc and 2 lcSSc subjects, all of whom we...
ORGANISM(S): Homo sapiens 
2015-03-25 | E-MEXP-1214 | biostudies-arrayexpress
TranscriptomicsThe Repertoire and Dynamics of Evolutionary Adaptations to Controlled Nutrient-Limited Environments in Yeast
The experimental evolution of laboratory populations of microbes provides an opportunity to observe the evolutionary dynamics of adaptation in real time.Until very recently, however, such studies have been limited by our inability to systematically find mutations in evolved organisms.We overcome thi...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-18 | E-GEOD-13435 | biostudies-arrayexpress
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