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ProteomicsMudPIT analyses of the human proteins biotinylated by proximity to biotin ligase (BioID) fused to the C-terminus of activators of the dynein/dynactin motor
To understand how a single motor achieves cargo specificity, we attached a promiscuous biotin ligase (BioID) to 6 distinct activators of the dynein machinery motile activity, including two new activators, ninein and ninein-like. BioID experiments were performed with stable HEK293 cell lines expressi...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-30 | MSV000080808 | MassIVE
ProteomicsMudPIT analyses of the human proteins biotinylated by proximity to exogenously expressed biotin ligase (BioID)
To identify non-specific biotinylation events, HEK293 cells transfected with a plasmid carrying only the biotin ligase moiety were used as negative controls to BioID experiments. Cells were lysed in the presence of detergents. After purification of biotinylated proteins on streptavidin-conjugated be...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-30 | MSV000080809 | MassIVE
ProteomicsLegionella pneumophila effector LegK3 phosphorylates multiple caspases involved in apoptosis signaling
Legionella pneumophila effector Legk3 phosphorylates caspase3/7/9 under infection, transfection, or in vitro purification
ORGANISM(S): Homo Sapiens 
2024-07-20 | PXD054088 |
ProteomicsLegionella pneumophila effector LegK3 directly phosphorylates multiple caspases involved in apoptosis signaling, including Caspase-3, Caspase-7, and Caspase-9
Legionella pneumophila effector Legk3 phosphorylates caspase3/7/9 under infection, transfection, or in vitro purification condition
ORGANISM(S): Homo Sapiens 
2024-07-28 | PXD054358 |
ProteomicsQuantitative proteomic analysis of Ricinus communis during early seed imbibition provided novel insights into cold stress response
Early planting is one of the strategies used to increase grain yield in temperate regions. However, poor cold tolerance in castor inhibits seed germination, resulting in lower seedling emergence and biomass. To date, proteomics studies have identified diverse proteins associated with germinating or ...
ORGANISM(S): Ricinus Communis 
2018-06-05 | PXD010043 |
ProteomicsDeepPhospho generated libraries, search results, models, and synthetic data
In this study, we present a hybrid deep neural network DeepPhospho which conceptually differs from all previous deep learning models for unmodified or modified peptide predictions in regard to peptide representation learning. Our approach utilizes a multi-module network and self attention mechanism ...
ORGANISM(S): Homo Sapiens 
2021-09-17 | PXD028601 |
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