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CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies
We show a recombinant lectin probe, rBC2LCN, is a new tool for fluorescence-based imaging and flow cytometry analysis of human pluripotent stem cells, as an alternative to conventional pluripotent maker antibodies. Live or fixed colonies of both human embryonic stem cells and induced pluripotent ste...
ORGANISM(S): Homo sapiens 
Visualizing and manipulating proteins in live cells is crucial for studying complex biological processes. Self-labelling protein (SLP) tags such as HaloTag and SNAP-tag can be fused to genes of interest to allow protein labelling in cells. New tools to enable rapid, specific and stable protein label...
ORGANISM(S): Homo sapiens (Human) 
2026-05-04 | PXD074567 | Pride
Transcription steps are marked by different modifications of the C-terminal domain of RNA polymerase II (RNAPII). Phosphorylation of Ser5 and Ser7 by cyclin-dependent kinase 7 (CDK7) as part of TFIIH marks initiation, whereas phosphorylation of Ser2 by CDK9 marks elongation. These processes are thou...
ORGANISM(S): Mus musculus 
Quantitative live-cell imaging of secretion activity reveals dynamic immune responses
Here we directly compare for the first time how the longstanding static model of mouse Dentate Gyrus (DG) development compares with a comprehensive high-resolution live-cell multiphoton (live-MPM) imaging approach. We took advantage of multiple fluorescent protein-based cell-type specific reporters ...
ORGANISM(S): Mus musculus 
Live-seq for live cell transcriptome profiling
Decoding the interplay between m6A modification and stress granule stability by live-cell imaging
To allow the study of unsaturated free fatty acids in live cells, we here report the use of sterculic acid, a 1,2-cyclopropene containing oleic acid analogue, as a bioorthogonal probe. We here show that this lipid can be readily taken up by dendritic cells without toxic side-effects, and that it can...
ORGANISM(S): Mus musculus (Mouse) 
2023-04-28 | PXD033831 | Pride
We introduce CALIPERS, a Fluorescence Ubiquitin Cell Cycle Indicator enabling cell cycle-aware phenotyping based on multiplexed live-cell imaging. We validate CALIPERS in three- and four-color reporter lines (HaCaT) and human induced pluripotent stem cells (WTC-11), co-expressing genetically encoded...
ORGANISM(S): Homo sapiens 
2026-06-25 | GSE281528 | GEO
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