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TranscriptomicsHigh-resolution transcriptome analysis with long-read RNA sequencing
Ongoing improvements to next generation sequencing technologies are leading to longer sequencing read lengths, but a thorough understanding of the impact of longer reads on RNA sequencing analyses is lacking. To address this issue, we generated and compared two RNA sequencing datasets of differing r...
ORGANISM(S): Homo sapiens 
2014-09-25 | E-GEOD-57862 | biostudies-arrayexpress
ProteomicsLong-read transcriptome sequencing reveals abundant promoter diversity in distinct molecular subtypes of gastric cancer
Deregulated gene expression is a hallmark of cancer, however most studies to date have analyzed short-read RNA-sequencing data with inherent limitations. Here, we combine PacBio long-read isoform sequencing (Iso-Seq) and Illumina paired-end short read RNA sequencing to comprehensively survey the tra...
ORGANISM(S): Homo sapiens (Human) 
2021-02-02 | PXD023373 | Pride
Isoform sequencing (iso-seq) Alternative promoters Gastric cancer Long-read sequencing 
TranscriptomicsComparative analysis of single-cell and spatial Nanopore long-read methods
Alternative splicing significantly contributes to transcriptome complexity and has critical implications for cellular functions. Recent advancements in single-cell isolation and capture techniques have enabled high-throughput quantification of gene expression at single-cell resolution. Long-read seq...
ORGANISM(S): Mus musculus 
2025-08-26 | E-MTAB-15190 | biostudies-arrayexpress
ProteomicsLong read proteogenomics to characterize protein isoform diversity in human umbilical vein endothelial cells (HUVECs)
Mass spectrometry-based proteomics sample preparation Harvested HUVECs, approximately 5 million cells each, were pelleted and frozen at -80C. The sample pellet was lysed according to the Filter Aided Sample Preparation (FASP) protocol.59 Lysis buffer used in the FASP was changed to 6% SDS, 150 mM DT...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-04-28 | MSV000089326 | MassIVE
TranscriptomicsCapTrap-Seq: A platform-agnostic and quantitative approach for high-fidelity full-length RNA transcript sequencing
Here we describe CapTrap-Seq, an experimental workflow designed to address the problem of reduced transcript end detection by long-read RNA sequencing methods, especially at the 5' ends. We apply CapTrap-Seq to profile transcriptomes of the human heart and brain and we compared the obtained results ...
ORGANISM(S): Mus musculus 
2023-12-20 | E-MTAB-13063 | biostudies-arrayexpress
ProteomicsA revised molecular model of ovarian cancer biomarker CA125 (MUC16) enabled by long-read sequencing
The biomarker CA125, a peptide epitope located in several tandem repeats of the mucin MUC16, is the gold-standard for monitoring regression and recurrence of high-grade serous ovarian cancer in response to therapy. However, the CA125 epitope along with several structural features of the MUC16 molecu...
ORGANISM(S): Homo sapiens (Human) 
2024-05-21 | PXD044117 | Pride
Proteomics Ovarian cancer Ca125 Biomarker Muc16 Nanopore sequencing 
GenomicsPk long read sequencing
Plasmodium knowlesi long read sequencing
PRJEB19298 | ENA
ProteomicsUsing long-read CAGE sequencing to profile cryptic-promoter derived transcripts and their contribution to the immunopeptidome
Recent studies have demonstrated that the non-coding genome can produce unannotated proteins as antigens that induce immune response. One major source of this activity is the aberrant epigenetic reactivation of transposable elements (TEs). In tumors, TEs often provide cryptic or alternate promoters,...
ORGANISM(S): Homo sapiens (Human) 
2023-09-23 | PXD040265 | Pride
Antigens Immunotherapy Epigenetic treatment Transposable elements Long-read sequencing 
TranscriptomicsPlate-based long-read single cell gene- and isoform transcriptome profiling using scLIS-seq
Single cell RNA-profiling in tandem with short-read sequencing (SR-scRNA-seq) has revolutionized the field of transcriptomics, permitting a highly granular view on cellular blood and tissue composition and the construction of human cell atlases. However, discrimination between various transcript iso...
ORGANISM(S): Homo sapiens 
2025-12-31 | E-MTAB-15047 | biostudies-arrayexpress
ProteomicsEnhancing the cardiac pig transcriptome by long-read sequencing and in-depth proteomics
Clinically translatable large animal models have become indispensable for cardiovascular research, clinically relevant proof of concept studies and for novel diagnostic and therapeutic interventions. In particular, the pig as emerged as an essential cardiovascular disease model, because its heart, c...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2020-10-29 | PXD018985 | Pride
Pig Tmt Annotation Proteomics Transcriptomics Cardiac Nanopore Lcms 
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