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ProteomicsLow pH Solid Phase Amino-Labeling of Complex Peptide Digests with TMTs Improves Peptide Identification Rates for Multiplexed Global Phosphopeptide Analysis
We present a novel Tandem Mass Tag Solid Phase Amino Labeling (TMT-SPAL) protocol using reversible immobilization of peptides onto octadecyl-derivatised (C18) solid supports. This method can reduce the number of steps required in complex protocols saving time and potentially reducing sample losses. ...
ORGANISM(S): Homo sapiens (Human) 
2015-04-21 | PXD002072 | Pride
Low ph solid phase amino-labeling Tmt Sysquant Spal 
OtherLow pH Solid Phase Amino-Labeling of Complex Peptide Digests with TMTs Improves Peptide Identification Rates for Multiplexed Global Phosphopeptide Analysis
Data from ProteomeXchange, PXD ID: PXD002072. Experiment: ppr140106, file: 20140212_ppr140106_Fr_02a.mzml. From ProteomeXchange: {{i}} We present a novel Tandem Mass Tag Solid Phase Amino Labeling (TMT-SPAL) protocol using reversible immobilization of peptides onto octadecyl-derivatised (C18) solid ...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM11210037672 | GPMDB
Resource Reanalysis 
TranscriptomicsDepletion of the Cullin Cdc53p Induces Morphogenetic Changes in Candida albicans
Transcriptional profiling of CDC53 down-regulated Candida albicans cells compared to control cells Keywords: comparative genomic hybridization, genetic modification Candida albicans is an important opportunistic human fungal pathogen, which can cause mucosal as well as systemic infections in immun...
ORGANISM(S): Candida albicans 
2010-05-14 | E-GEOD-13976 | biostudies-arrayexpress
ProteomicsProteome-wide diet-independent adipose tissue alterations link dendritic cells to 2-aminoadipoate associated insulin sensitivity.
Adipose tissue dysfunction in obese humans is associated with disrupted metabolic homeostasis, insulin resistance, and type 2 diabetes mellitus (T2DM). In a mouse model that has preserved insulin sensitivity despite increased adiposity, we used unbiased three-dimensional integration of proteome prof...
ORGANISM(S): Mus musculus (Mouse) 
2018-08-06 | PXD008660 | Pride
Insulin resistance Isobaric labeling Adipose tissue biology Inflammation Extended multiplexing Branched chain amino acid metabolism Quantitative proteomics Obesity 
TranscriptomicsIGR_GLIOMA_STUDY_SP
Glioma study (gene expression and CGH): Brain tumours are the most common solid tumors in children and have the highest mortality rate of all solid pediatric tumours. Despite advances in multimodality therapy, children with high-grade gliomas invariably have an Overall Survival (OS) around 20% at 5 ...
ORGANISM(S): Homo sapiens 
2012-02-22 | E-TABM-1107 | biostudies-arrayexpress
ProteomicsChronic alcohol consumption reprograms hepatic metabolism through organelle-specific acetylation in mice.
Post-translational acetylation of proteins at lysine side chains by the central metabolite acetyl-CoA, is a crucial regulator of proteostasis. Ethanol metabolism in the liver induces protein acetylation and disrupts hepatic substrate metabolism. While acetylation can influence gene transcription, en...
ORGANISM(S): Mus musculus (Mouse) 
2025-08-04 | PXD055349 | Pride
Proteomics Acetylation Liver Alcohol Lc-ms/ms 
ProteomicsExtended multiplexing of TMT labeling reveals age and high fat diet specific proteome changes in mouse epididymal adipose tissue
Age-related and/or high caloric intake driven changes in visceral adipose tissue contribute to the development of diabetes and cardiovascular disease. The lack of reliable, high-throughput methods to analyze the adipose tissue protein composition has limited our understanding of the protein network...
ORGANISM(S): Mus musculus (Mouse) 
2017-03-27 | PXD005953 | Pride
Protein expression Low fat diet Mudpit Proteomics Internal reference scaling normalization High fat diet Mouse Extended multiplexing Epididymal adipose tissue Aging Tandem mass tags Orbitrap fusion 
ProteomicsHyperSCP: Combining Isotopic and Isobaric Labeling for Higher Throughput Single-Cell Proteomics
Recent developments in mass spectrometry-based single-cell proteomics (SCP) have resulted in dramatically improved sensitivity, yet the relatively low measurement throughput remains a limitation. Isobaric and isotopic labeling methods have been separately applied to SCP to increase throughput throug...
ORGANISM(S): Homo sapiens (Human) 
2023-07-11 | PXD040455 | Pride
Multiplex Single-cell proteomics High-throughput 
TranscriptomicsTime course of Streptomyces coelicolor: M145 Control vs. wblA deletion mutant
The basic experiment is a comparison of gene transcript levels of Streptomyces coelicolor M145 and its wblA deletion mutant at 6 timepoints (24, 36, 48, 60, 72 and 84 hours) encompassing vegetative growth, aerial growth, and sporulation on solid medium. The wblA mutant is morphologically defective, ...
ORGANISM(S): Streptomyces coelicolor 
2011-01-28 | E-GEOD-24396 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human monocytic cells THP-1 stimulated with LPS (10ng/ml) in presence or absence of LL-37 (5 ug/ml), as well as with the peptide alone, for 4 hours
The objective of the study was to evaluate transcriptional response of endotoxin-stimulated human monocytic cells in presence or absence of host defense peptide LL-37 at low physiologically relevant concentrations. Human monocytic cells THP-1 were stimulated with LPS (10ng/ml) in presence or absenc...
ORGANISM(S): Homo sapiens 
2006-12-07 | E-FPMI-6 | biostudies-arrayexpress
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