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TranscriptomicsRNA-seq of small intestinal organoids treated with an inhibitor for the epigenetic modifier LSD1
To assess the role of LSD1 in mice small intestinal epithelium, small intestinal organoids were treated with an inhibitor for LSD1 (GSK-LSD1) and compared to untreated organoids. Similar to intestinal epithelium from mice with an intestinal epithelium specific LSD1-KO, paneth cells dissappear upon G...
ORGANISM(S): Mus musculus 
2020-11-16 | E-MTAB-9077 | biostudies-arrayexpress
TranscriptomicsHuman small intestinal organoids grown with a specific protocol to enrich for Paneth cells were treated with an inhbitor (GSK-LSD1) for the epigenetic modulator LSD1.
To assess the role of LSD1 in human intestinal epithelium, human small intestinal organoids were treated with an inhibitor for LSD1 (GSK-LSD1) and compared to untreated organoids. The organoids were grown with specific conditions where Paneth cells are present in the organoids as similar experiments...
ORGANISM(S): Homo sapiens 
2020-11-16 | E-MTAB-7863 | biostudies-arrayexpress
GenomicsGenome-wide map of lysine specific demethylase 1 (LSD1) in mouse embryonic stem cells.
We report the identification of LSD1 binding genomic regions in mouse embryonic stem cells (ESC) by high throughput sequencing. By obtaining over 10 million 36 bp reads of sequence from each chromatin immunoprecipitated DNA, we generated genome-wide maps for LSD1 and histone H3 dimethylated on lysin...
ORGANISM(S): Mus musculus 
2012-10-03 | E-GEOD-18515 | biostudies-arrayexpress
GenomicsGenome-wide maps of Lsd1 binding sites in escort cells/early follicle cells and cap cells/terminal filament cells
Loss of Lsd1 in Drosophila in specific cells of the Drosophila ovary results in increased BMP signaling outside the cap cell niche and an expanded germline stem cell (GSC) phenotype. To better characterize the function of Lsd1 in different cell populations within the ovary, we performed Chromatin im...
ORGANISM(S): Drosophila melanogaster 
2014-02-15 | E-GEOD-54376 | biostudies-arrayexpress
ProteomicsLSD1 interactome in Glioblastoma
Tumor-initiating cells (TICs) play a critical role in glioblastoma (GBM) maintenance being responsible for its heterogeneity and resistance to standard therapy. A step toward clinical translation includes GBM TIC targeting. Among the molecules tested for GBM treatment, are those targeting epigeneti...
ORGANISM(S): Homo sapiens (Human) 
2022-06-02 | PXD021429 | Pride
Glioblastoma Lsd1 Protein complex Lsd1 inhibitor Targeted therapy 
ProteomicsMudPIT analyses of the proteins co-purified with components of the LSD1 (KDM1A) transcriptional repressor complex and the non-canonical BAF complex, immuno-precipitated from MKL-1 and WaGa MCC cell lines
Protein Complexes Purification: Immunoprecipitations were performed with antibodies to LSD1 (2139; Cell signaling technology), INSM1 Mouse (SC-271408, Santa Cruz Biotechnology), RCOR2 (23969-1-AP; Proteintech Group), BRD9 (61537; Active Motif Rabbit), and normal Rabbit IgG (2729; Cell signaling tech...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-01-25 | MSV000083364 | MassIVE
TranscriptomicsLNCaP and C42B LSD1 knockdown microarray gene expression data and C42B androgen (DHT) stimulation microarray gene expression data
LSD1 (also known as KDM1A) is a histone demethylase and a key regulator of gene expression in embryonic stem cells and cancer. LSD1 was initially identified as a transcriptional repressor via its demethylation of active histone H3 marks (di-methyl lysine 4 [2MK4]). In prostate cancer, specifically, ...
ORGANISM(S): Homo sapiens 
2016-06-30 | E-GEOD-61630 | biostudies-arrayexpress
GenomicsChIP-sequencing of the H3K4me1/2 in crypts from mice intestinal epithelium with intestinal epithelium specific LSD1 knock out compared to wild type
To study the epigenetic regulation of intestinal epithelium we focus on the role of chromatin modulators. Lysine-specific histone demethylase 1a (KDM1A, LSD1) is one of the enzymes that can erase the H3K4me1/2 mark. To assess the role of LSD1 in intestinal epithelium we studied wild type (WT) (Villi...
ORGANISM(S): Mus musculus 
2020-11-16 | E-MTAB-7871 | biostudies-arrayexpress
TranscriptomicsRNA-seq of crypt cells from mouse small intestinal epithelium with an intestinal-epithelial specific deletion of LSD1 compared to wild type crypts
To assess the role of LSD1 in mouse small intestinal epithelium, we isolated small intestinal crypts from wild type (WT) (Villin-Cre -; Lsd1f/f) and intestinal-epithelial-specific knock-out (KO) (Villin-Cre+; Lsd1f/f) mice. We dissociated crypts into single cells, and FACS sorted Epcam+ cells, to av...
ORGANISM(S): Mus musculus 
2020-11-16 | E-MTAB-7862 | biostudies-arrayexpress
TranscriptomicsTargeting Group3 Medulloblastoma by the anti-PRUNE-1 and anti-LSD1- KDM1A molecules augments their potential therapeutic value.
The combination of AA7.1 and SP2577 affects neuronal commitment,leukocyte differentiation and mitochondrial metabolism. To better dissect the mechanisms of action of these two drugs and to investigate the genes and pathways affected we treated primary Group3 MB for 24 hours with 100μM AA7.1 (prunei...
ORGANISM(S): Homo sapiens 
2024-03-30 | E-MTAB-13616 | biostudies-arrayexpress
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