Sort   by:  
 Page size 
Proteomicscoimmunoprecipitation of BdRFS identifies putative itneracting proteins
In this work we sought to identify putative interacting proteins of the Brachypodium distachyon Regulator of Stress and Flowering protein (BdRFS) from healthy leaf tissue. A truncated form of BdRFS lacking the N-terminal 45 residues was necessary for solubilization. THe protein is tagged N-terminall...
ORGANISM(S): Brachypodium distachyon (Purple false brome) (Trachynia distachya) 
2023-03-11 | PXD033835 | Pride
Rfs Brachypodium 
ProteomicsHeterologous Expression of MBP-AtABC1K6-His in E. coli
Heterologous E. coli expression of AtABC1K6 tagged with maltose binding protein (MBP) and 6xHis tags revealed a spontaneous cleavage event of the recombinant protein during purification. Resulting bands were resolved on SDS-PAGE and excised for MS/MS identification. Mass spec-based identification of...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-03-02 | PXD030384 | Pride
Coq8 Kinase Spontaneous cleavage Ubib Abc1 
ProteomicsPlastoglobules BN-PAGE experiment (Arabidopsis PGs under unstress and high light conditions)
Proteome comparison of isolated plastoglobules (PGs) from Arabidopsis thaliana (Col-0 ecotype) under unstress (UNS) and high light (HL) stress conditions. For both conditions, PGs were isolated from three independent set of plants each (i.e. three for UNS and three for HL). PGs were isolated from th...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-04-13 | PXD019735 | Pride
Arabidopsis thaliana Light stress Plastoglobules Orbitrap 
ProteomicsArabidopsis plastoglobules 2D-gel separation
Protein complexes separation of isolated plastoglobules (PGs) from Arabidopsis thaliana (Col-0 ecotype). PGs were isolated from the plants when they were 4 weeks old under long day photoperiod ( during the morning in dark adapted plants,16 hours of light and 8 darkness photoperiod, LED light of 120 ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-04-13 | PXD019765 | Pride
Pgs Arabidopsis 
ProteomicsShotgun Proteomics of BN-PAGE-Resolved Chloroplasts
Blue Native-PAGE resolves protein complexes in their native state. When coupled with immunoblotting, it can be used to identify the presence of high molecular weight complexes at high resolution for any protein, given a suitable antibody. To identify proteins in high molecular weight complexes on a ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-12-23 | PXD003162 | Pride
Oligomeric state Native gel Protein complex Native molecular weight Chloroplast 
Sort   by:  
 Page size