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ProteomicsDIA-PASEF–based FLAG-IP proteomic analysis of nuclear or cytosolic p62 condensates in HeLa cells
Cells were cross-linked with 0.1% formaldehyde for 10 min at 25 °C and quenched with 100 mM glycine. After washing with HBS (20 mM HEPES-NaOH, pH 7.5, 150 mM NaCl), cells were lysed in HEPES-RIPA buffer (20 mM HEPES-NaOH, pH 7.5, 150 mM NaCl, 1 mM MgCl₂, 1 mM EGTA, 1% NP-40, 0.25% sodium deoxycholat...
ORGANISM(S): Homo Sapiens (human) 
2025-12-30 | PXD072519 | JPOST Repository
ProteomicsDIA-PASEF–based total proteome profiling of HeLa cells expressing nuclear or cytosolic p62 condensates
Cells were lysed in 6 M guanidine-HCl, 100 mM HEPES-NaOH, pH7.5, 10 mM TCEP, and 40 mM CAA. The lysates were dissolved by heating and sonication, followed by centrifugation at 20,000 × g for 15 min at 4 °C. The supernatants were recovered, and proteins (20 µg each) were purified by the SP3 method an...
ORGANISM(S): Homo Sapiens (human) 
2025-12-30 | PXD072518 | JPOST Repository
ProteomicsPhosphoproteome and proteome of mouse hepatocyte maturation and human hepatic reprogramming
To identify central protein kinases that potentially promote the maturation, we chemically induced liver progenitor cells (CLiPs) from mouse hepatocytes using a previously established protocol, and profiled the phosphoproteome and proteome of freshly isolated primary mouse hepatocytes (MHs) and ALBU...
ORGANISM(S): Homo Sapiens Mus Musculus 
2022-08-05 | PXD035829 |
ProteomicsNociceptive neurons inhibit neutrophil extracellular trap formation via MLKL-licensed histone release
This data set contains the raw data of proteinomic analysis of dorsal root ganglions (DRGs) in WT and MLKL-KO mice under basal condition and after CFA treatment, and MLKL-based IP-MS analysis of WT DRGs under basal condition and after CFA treatment.
ORGANISM(S): Mus Musculus 
2026-04-16 | PXD077273 |
ProteomicsPSAT1 impairs ferroptosis and reduces immunotherapy efficacy via GPX4 hydroxylation
MDA-MB-231 cells with expression of Flag-GPX4 were treated with IFNg. An immunoprecipitation assay was performed, and immunoprecipitates were subjected to masspec analysis.
ORGANISM(S): Homo Sapiens 
2025-03-01 | PXD061373 |
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