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Here we present a microfluidics-based affinity purification-mass spectrometry (AP-MS) pipeline to identify protein-protein interactions (PPI) using minute amounts of input material. The use of this automated platform allowed us to identify the human cohesin and CCC complex from only 4 micrograms of ...
ORGANISM(S): Homo sapiens (Human) 
2022-03-10 | PXD011323 | Pride
Experiments conducted on this tiling array are used to (1) validate the frozen gene sets of the current genome annotation, (2) improve the predicted gene structures by empirically determining UTRs and intron-exon boundaries, identifying missing upstream, internal, and downstream exons and alternativ...
ORGANISM(S): Daphnia pulex 
Essentially all cellular processes are orchestrated by protein-protein interactions (PPIs). In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular PPIs. Here we present a microfluidics-based (AP-MS) workflow to identify PPIs usi...
ORGANISM(S): Homo sapiens (Human) 
2019-04-02 | PXD012800 | Pride
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