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UnknownCell Lysate-Based AlphaLISA Deubiquitinase Assay Platform for Identification of Small Molecule Inhibitors.
Not available
S-EPMC5947317 | biostudies-literature
ProteomicsDifferential kinobeads selectivity profiling
The goal of this project was to use differential kinobeads profiling for the discrimination of reversible and irreversible kinase targets of different covalent kinase inhibitors. For this, differences in target engagement measured in a lysate- and a cell-based kinobeads assay were compared in a kino...
ORGANISM(S): Homo sapiens (Human) 
2017-10-13 | PXD007920 | Pride
Ibrutinib Kinobeads Ramos b-lymphocyte Cell-based assay 5z-7-oxozeaenol Saturated ibrutinib A549 lung epithelia cell line Lysate-based assay Acalabrutinib Cc-292 Lc-ms/ms 
UnknownInvestigating and Optimizing the Lysate-Based Expression of Nonribosomal Peptide Synthetases Using a Reporter System.
Not available
S-EPMC11236431 | biostudies-literature
ProteomicsSWATH mass spectrometry performance using extended ion assay libraries
The use of data-independent acquisition methods such as SWATH for mass spectrometry based proteomics is usually performed using peptide MS/MS reference ion assay libraries which enable identification and quantitation of peptide peak areas. Reference assay libraries can be generated locally through ...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2016-05-12 | PXD002836 | Pride
Extended library Dia Swath 
ProteomicsA comprehensive spectral assay library to quantify the Halobacterium salinarum NRC-1 proteome by DIA/SWATH-MS
Data-Independent Acquisition (DIA) is a mass spectrometry-based method to reliably identify and reproducibly quantify large fractions of a target proteome. The peptide-centric data analysis strategy employed in DIA requires a priori generated spectral assay libraries. Such assay libraries allow to e...
ORGANISM(S): Halobacterium salinarum 
2023-09-21 | PXD042770 | Pride
Dia Data-independent acquisition Swath Halobacterium salinarum nrc-1 Spectral assay library Dda Data-dependent acquisition 
TranscriptomicsTranscription profiling of leukemia samples prepared using different RNA isolation procedures
Microarray gene expression (MAGE) signatures allow insights into the transcriptional processes of leukemias and may evolve as a molecular diagnostic test. Introduction of MAGE into clinical practice of leukemia diagnosis will require comprehensive assessment of variation due to the methodologies. Fo...
ORGANISM(S): Homo sapiens 
2008-11-06 | E-GEOD-7757 | biostudies-arrayexpress
UnknownFluorescence dequenching assay for the activity of TEV protease.
Not available
S-EPMC9662696 | biostudies-literature
UnknownFluorescent probe for high-throughput screening of membrane protein expression.
Not available
S-EPMC3832049 | biostudies-literature
UnknownMultiplexing fluorogenic esterase-based viability assay with luciferase assays.
Not available
S-EPMC6812399 | biostudies-literature
ProteomicsCobB interacting proteins identified by SPIDER assay
To assess whether SPIDER could detect transient interactions such as enzymes and their substrates, we examined the interactome of the E. coli protein deacetylase CobB. As the only member of the Sir2 family of deacetylases in E. coli, CobB is known to play a role in many different pathways but their ...
ORGANISM(S): Escherichia Coli 
2021-06-04 | PXD026514 |
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