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RNA m5C methylation profile of MCF10A and MDA486 by using MeRIP-Seq protocol Immunoprecipitation of Methylated mRNA at Cytosine (m5C) residues: Affinity purified of anti-methyl cytosine (m5C) polyclonal antibody 7ug (Zymo Research, Catalog#A3001-50) was conjugated with protein-A magnetic beads for 2...
ORGANISM(S): Homo sapiens 
Protocol for detecting m5C RNA modification at single-base resolution using m5C-TAC-seq
m5C-MeRIP-seq of HHL5 cells
TET2 regulates histone H2AK119ub and leukemogenesis through LTR RNA m5C oxidation [seq_RNA-caRNA-m5C-MeRIP]
The biotin labeled DNA:RNA hybrids with or without RNA m5C modification were incubated with lysates of 293 cells treated with H2O2, and were pulled down by Streptavidin. The proteins pulled down by the empty beads control, unmodified or m5C-modified hybrids were analyzed by mass spectrometry
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-04-29 | MSV000089337 | MassIVE
Transcriptome m5C MeRIP-seq of human Huh7 cells
The field of epitranscriptomics is growing in importance, with chemical modification of RNA being associated with a wide variety of biological phenomena. Mass spectrometry (MS) enables the identification of modified RNA residues within their sequence contexts, by using analogous approaches to shotgu...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-31 | PXD016308 | Pride
The field of epitranscriptomics is growing in importance, with chemical modification of RNA being associated with a wide variety of biological phenomena. Mass spectrometry (MS) enables the identification of modified RNA residues within their sequence contexts, by using analogous approaches to shotgu...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-12-31 | PXD012095 | Pride
m5C MeRIP sequencing in PANC-1 cells to map the m5C methylome of PANC-1 cells treated with CAF-derived Evs
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