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TranscriptomicsMechanisms that specify promoter nucleosome location and identity
The chromatin architecture of eukaryotic gene promoters is generally characterized by a nucleosome-free region (NFR) flanked by at least one H2A.Z variant nucleosome. Computational predictions of nucleosome positions based on thermodynamic properties of DNA-histone interactions have met with limite...
ORGANISM(S): Saccharomyces cerevisiae 
2009-04-30 | E-GEOD-13446 | biostudies-arrayexpress
TranscriptomicsHypoxia response in C. neoformans in H99, CM092 and CM098
(1) The identification of genes that are transcriptionally regulated in response to exposure to hypoxia (~0.2% oxygen) in H99 (WT) and strains CM092 and CM098. (2) The identification of genes that are differentially-regulated in the strain CM093 compared to H99 (WT) Keywords: hypoxia response, compa...
ORGANISM(S): Filobasidiella neoformans 
2010-06-12 | E-GEOD-6226 | biostudies-arrayexpress
TranscriptomicsSilencing factors induce the elimination of nucleosome-free regions in S. pombe heterochromatin
Abstract: In the yeast S. pombe, multiple chromatin-modifying enzymes are required for heterochromatin formation, yet how their actions alter chromatin structure to block access to DNAby the transcriptional machinery is unknown. We constructed high-resolution nucleosome occupancy maps of heterochrom...
ORGANISM(S): Schizosaccharomyces pombe 
2010-11-24 | E-GEOD-19596 | biostudies-arrayexpress
ProteomicsProximity labelling and LCMSMS analysis of spliceosome complexes using SUGP1-miniTurbo
The purpose of the project is to identify proteins binding differentially to the human specific spliceosomal protein SUGP1 in the context of point mutations to its G-patch domain. SUGP1 wt and mutant (G603N or L570E) C-terminally tagged with mini-Turbo-NLS was overexpressed for 48 h in HEK293T cell...
ORGANISM(S): Homo sapiens (Human) 
2023-10-05 | PXD038067 | Pride
Spliceosome Sugp1 Proximity labeling Miniturbo Bioid 
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