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ProteomicsProteome profiling of enriched lysosomes obtained from four commercial isolation methodologies.
Lysosomes are membrane-bound cell organelles that can be found in all mammalian cells. They contain digestive enzymes and are involved in multiple processes, including degradation of worn-out cell parts, antigen presentation and induction of programmed cell death. Mass spectrometry studies of lysoso...
ORGANISM(S): Homo sapiens (Human) 
2026-02-23 | PXD040003 | Pride
Tmt Lysosome Sp3 Thp-1 Organelle enrichment 
Proteomicslysosome proteomics in Niemann-Pick Type C disease model cells (CHOwt and NPC1-null cells)
Increasing evidence implicates lysosomal dysfunction in the pathogenesis of neurodegenerative diseases, including the rare inherited lysosomal storage disorders (LSDs) and the most common neurodegenerative diseases, such as Alzheimer’s and Parkinson’s disease (AD and PD). The goal of this work was t...
ORGANISM(S): Homo sapiens (Human) 
2018-03-21 | PXD008438 | Pride
Magnetic nanoparticles lysosome enrichment Npc1-null cells Chowt cells Lysosome Lc-ms/ms 
ProteomicsTwo step enrichment facilitates background reduction for proteomic analysis of lysosomes
Lysosomes comprise the main degradative compartment of almost all mammalian cells and are involved in various cellular functions, most of which are catalyzed by the lysosomal proteome. Lysosomal proteins are low abundant, complicating their analysis by mass spectrometry-based proteomics. To increase...
ORGANISM(S): Homo sapiens (Human) 
2024-08-01 | PXD048696 | Pride
Lysosomes  lysosome enrichment  superparamagnetic iron oxide particles (spions)  tmem192 Mass spectrometry  proteomics  organelles Hek 293 
ProteomicsNovel surface functionalized superparamagnetic nanoparticles with engineered cellular uptake as a means for intracellular omics
Superparamagnetic nanoparticles (SPMNPs) are appealing for use in organelle isolation strategies. Yet, this potential remains largely unexplored because thus far research has focused on either physicochemical design or on their application in micron-sized beads. For their use in life sciences, the b...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-28 | MSV000080688 | MassIVE
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