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ProteomicsHuman plasma proteins detected by HPLC-MS/MS after blood incubation with E. coli treated/not treated with mucin
Whole human blood incubated with E. coli isolate with or without bound mucin. Plasma was obtained and the effect of mucin bound to E. coli on plasma proteins content was analysed by HPLC-MS-MS.
ORGANISM(S): Homo sapiens (Human) 
2019-12-16 | PXD010496 | Pride
Plasma Mucin E. coli 
ProteomicsMucin-binding proteins of E. coli membrane
Proteins from E. coli outer membrane were extracted with carbonate method, separated in 2D-PAGE and transferred onto nitrocellulose membrane. After incubation with flourescent-labelled mucin, mucin-binding protein spots were cut out, subjected to trypsinolysis and identified by protein analysis wit...
ORGANISM(S): Escherichia coli 
2019-12-16 | PXD010472 | Pride
Escherichia coli Hplc-ms-ms Mucin binding Membrane 
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