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We present data from tissues of seven oesophageal adenocarcinomas of CD4 and CD8 T cell epitopes eluted from the cell surface using mass spectrometry (immunopeptidomics) of presented HLA bound peptides.This dataset forms part of the publication:Nicholas, B., Bailey, A., McCann, K.J., Wood, O., Walke...
ORGANISM(S): Homo sapiens (Human) 
2022-09-27 | PXD031108 | Pride
Samples were reduced in loading buffer and separated by SDS-PAGE for 3-4 min followed by in-gel digestion with trypsin. Digests were analyzed by LC-MS/MS using a Waters nanoACQUITY interfaced to a Thermo Fusion Lumos MS. The LC separation 90 min gradient of 5-30% MeCN in a trapping configurations w...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-12-30 | MSV000086651 | MassIVE
We investigated differences in the chromatin-binding profile of SOX18 (mouse) and its dominant-negative isoform SOX18RaOp, and how SOX18RaOp alters the binding location of SOX18. HeLa cells were transfected to generate 3 different conditions: 1. myc-SOX18, 2. myc-SOX18RaOp and 3. myc-SOX18 with unta...
ORGANISM(S): Homo sapiens 
Cortical injury frequently causes motor dysfunction, significantly impairing activities of daily living, and currently, there are no effective therapeutic options available. Previous studies demonstrated that intravenous infusion of mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) e...
ORGANISM(S): Macaca mulatta (Rhesus macaque) 
2026-06-15 | PXD059286 | Pride
Protein concentrations of both lysed WBC and plasma samples were measured by the PierceTM Bradford Assay and normalized to contain 10 μg per sample before proceeding with sample digestion using the procedure as previously described44. Peptides were desalted using STop-And-Go Extraction tips (STAGE t...
ORGANISM(S): Homo sapiens (Human) 
2020-12-01 | PXD020474 | Pride
Comparative proteomics of ER-Golgi membranes from etiolated coleoptiles of maize (Zea mays) and young leaves of Arabidopsis (Arabidopsis thaliana) isolated by flotation centrifugation demonstrate differences in the complement of synthases and glycosyl transferases unique to the type of wall made. Ad...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Zea mays (Maize) 
2019-04-11 | PXD007612 | Pride
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