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TranscriptomicsRNA-Seq of Ras induced Epithelial to Mesenchymal Transition (EMT) in Madin-Darby Canine Kidney (MDCK) cells
To gain more information about EMT induced by oncogenic Ras in MDCK cells, we performed RNA-seq of MDCK and MDCK-Ras cells and compared their genome-wide gene expression profiles.
ORGANISM(S): Canis lupus familiaris 
2016-02-03 | E-MTAB-3301 | biostudies-arrayexpress
TranscriptomicsMadin-Darby Canine Kidney (MDCK) cells grown in 2-dimensional and 3-dimensional type I collagen culture.
In order to gain insight into epithelial morphogenesis and the influence of culture geometry on gene expression patterns, Madin Darby Canine Kidney (MDCK) epithelial cells where grown in 2-dimensional (2D) culture or 3-dimensional (3D) culture . MDCK cells cultured in 2D were plated atop a pre-soli...
ORGANISM(S): Canis lupus familiaris 
2013-06-03 | E-GEOD-28381 | biostudies-arrayexpress
TranscriptomicsExpression data from MDCK cells exposed to HGF +/- MAPK inhibitors
Gene expression in Madin Darby canine kidney cells grown for 7 days to confluence on Transwell filters and exposed to HGF +/- inhibitors of the MAPK pathway (MAPK is one of the pathways activated when HGF binds to the CMET receptor tyrosine kinase). We used microarrays to detail the program of gene ...
ORGANISM(S): Canis lupus familiaris 
2010-05-25 | E-GEOD-9435 | biostudies-arrayexpress
TranscriptomicsGene expression of normal MDCK cells mixed with normal MDCK cells or RasV12-transformed MDCK cells
To investigate whether and how the presence of RasV12-transformed cells influences the gene expression profile of the neighbouring normal epithelial cells, we performed microarray analysis. Normal Madin-Darby canine kidney (MDCK) cells were co-cultured with MDCK cells expressing GFP or GFP-RasV12. G...
ORGANISM(S): Canis lupus familiaris 
2020-03-10 | E-MTAB-8769 | biostudies-arrayexpress
TranscriptomicsRNA-seq of canine MDCK epithelial cell line compared to cell line overexpressing ERK2 or cell line with conditional activation of the ERK pathway
Using RNA-seq and canine MDCK epithelial cells, we analyzed mRNA expression profiles of cells overexpressing ERK2 and cells where the ERK pathway was activated for 24 hrs. For this purpose, three cell lines were used: parental MDCK cells, MDCK cells overexpressing FLAG-ERK2 or MDCK cells expressing ...
ORGANISM(S): Canis lupus familiaris 
2022-10-01 | E-MTAB-12132 | biostudies-arrayexpress
TranscriptomicsmicroRNA-Seq of Ras induced Epithelial to Mesenchymal Transition (EMT) in Madin-Darby Canine Kidney (MDCK) cells
To gain more information on the expression of microRNAs during EMT induced by oncogenic Ras in MDCK cells, we performed deep sequencing (Illumina HiSeq2000) of MDCK and MDCK-Ras cells and compared their genome-wide microRNA expression profiles. RNA-Seq data from the same study has also been deposite...
ORGANISM(S): Canis lupus familiaris 
2015-11-17 | E-MTAB-3302 | biostudies-arrayexpress
TranscriptomicsE-cadherin and EMT regulation by E47 transcription represor
Expression profile analysis in MDCK-E47 cells in comparisom with MDCK CMV control cells Keywords: Genetic Modification (overexpression of E-cadherin repressors) The Oncochip microarray platform v1.1 contains 9,726 clones corresponding to 6,386 different genes, and it includes 2,489 duplicate clones....
ORGANISM(S): Canis lupus familiaris 
2010-06-24 | E-GEOD-9147 | biostudies-arrayexpress
TranscriptomicsE-cadherin and EMT regulation by bHLH factors: a new role for the class I gene E2-2 (TCF4) and its comparison with E47.
Loss of expression of the cell-cell adhesion molecule E-cadherin is an essential event for epithelial-mesenchymal transition (EMT), a process that allows cell migration in the developing embryo and during tumour invasion. Transcriptional repression has emerged as the main mechanism responsible for E...
ORGANISM(S): Canis lupus familiaris 
2010-06-24 | E-GEOD-9145 | biostudies-arrayexpress
TranscriptomicsMadin Darby Canine Kidney (MDCK) Cells: Early & Late Passage
The paracellular passage of ions in epithelial systems is dictated by the repertoire of tight junction (TJ) proteins, the number and architecture of apicolateral TJ strands, and the interaction of TJ proteins with the cytoskeleton. To investigate the relative contribution of these factors and to ex...
ORGANISM(S): Canis lupus familiaris 
2010-04-09 | E-GEOD-14837 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis of MDCK parental cells (ATCC vs ECCAC) in response to CAV2 infection.
Phenotypic variability in cultured mammalian cell lines has long been recognized and known to be driven by passaging and culture conditions. Yet, the effect these variations have on the production of gene therapy viral vectors has been largely overlooked. In this work we evaluated the impact of usin...
ORGANISM(S): Canis lupus familiaris 
2020-07-31 | E-MTAB-9379 | biostudies-arrayexpress
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