Sort   by:  
 Page size 
ProteomicsQuantitative evaluation of FASP and MED FASP protocols
In this work we re-evaluate FASP and the related Multienzyme digestion (MED)-FASP method. We use different types of animal tissue and cultured cells, and test the performance of the method under various conditions. We analyze the protein-to-peptide conversion by assessing the yield of peptides, freq...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2016-04-28 | PXD003733 | Pride
Fasp  med fasp  missing cleavages  reproducibility 
ProteomicsOptimization of quantitative proteomic analysis of clots
The aim of our study was to optimize quantitative proteomic analysis of fibrin clots prepared ex vivo from citrated plasma of the peripheral blood drawn from patients with prior venous thromboembolism. We used a multiple enzyme digestion filter aided sample preparation (MED FASP) method combined wi...
ORGANISM(S): Homo sapiens (Human) 
2017-12-08 | PXD006814 | Pride
Proteomics Venous thromboembolism Clot Med fasp 
ProteomicsD283 MED, a cell line derived from peritoneal metastatic medulloblastoma — a good choice for missing protein discovery
Our goal is the identification of more and more missing proteins from cancer cell lines. This project focuses on D283 med and U-118mg cell lines which contribute to 12 missing protein identification.
ORGANISM(S): Homo sapiens (Human) 
2020-11-26 | PXD021482 | Pride
Missing protein  lc-msms  d283 med cell  hpp 
ProteomicsSensitivity and protein digestion course of proteomic Filter Aided Sample Preparation
1. Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. We found that FASP allows effective processing samples at the low and sub-microgram range. 2. Whole cell lysates of Hela cells were processed with FASP using...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-11-12 | PXD014288 | Pride
Fasp Sample preparation 
ProteomicsComparative analysis of mouse muscles types
The dataset presents FASP- and LC-MS/MS-based analysis of whole lysates of tree types of mouse muscle tissue: white and red muscle fibers and heart muscle. The measurements were performed using five biological replicates for each tissue type. The dataset cover 7200 proteins. The data were used for ...
ORGANISM(S): Mus musculus (Mouse) 
2016-06-21 | PXD002152 | Pride
Skeletal muscle proteome Metabolism Quantitative proteomics Protein titer 'total protein approach' Glycolysis Heart muscle proteome Med fasp 
ProteomicsComparison of proteome composition of serum enriched in extracellular vesicles isolated from polycythemia vera patients and healthy controls
Extracellular vesicles (EVs), e.g., exosomes and microvesicles, are one of the main networks of intercellular communication. In myeloproliferative disorders, such as polycythemia vera (PV), excess of EVs originating from overabundant blood cells can directly contribute to thrombosis through their pr...
ORGANISM(S): Homo sapiens (Human) 
2019-05-13 | PXD013234 | Pride
Human plasma Extracellular vesicles Vesicles 
ProteomicsQuantitative Analysis of of Human Liver
Large scale proteomic analysis of human liver from 10 donors
ORGANISM(S): Homo sapiens (Human) 
2018-04-23 | PXD003458 | Pride
Human liver  total protein approach  med-fasp  fasp 
ProteomicsLeaf protein extraction method
We evaluated several approaches for sample processing that are based on popular urea extraction, methanol/chloroform, and phenol methods. Additionally, we examined whether these methods were improved using FASP-based on filter digestion. We found that phenol-FASP and UA-FASP methods enabled the bes...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2018-02-20 | MSV000082080 | MassIVE
ProteomicsArabidopsis Root Protein Extraction Evaluation
We evaluated several approaches for sample processing that are based on popular urea extraction, methanol/chloroform, and phenol methods. Additionally, we examined whether these methods were improved using FASP-based on filter digestion. We found that phenol-FASP and UA-FASP methods enabled the best...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2018-05-15 | MSV000082362 | MassIVE
UnknownOptimization of quantitative proteomic analysis of clots generated from plasma of patients with venous thromboembolism.
Not available
S-EPMC5706328 | biostudies-literature
Sort   by:  
 Page size