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MetabolomicsIntegrated proteome and lipidome analyses place OCIAD1 at mitochondria-peroxisome intersection balancing lipid metabolism

OCIAD1 (Ovarian Cancer Immunoreactive Antigen Domain Containing 1) is a membrane protein largely localized to mitochondria, however, its function in health or disease is not well understood. To comprehensively characterize the molecular changes upon lack of OCIAD1, we used mass spectrometry to st...

2025-04-25 | MTBLS12329 | MetaboLights
MetabolomicsThe Impacts of Sortase A and the 4'-Phosphopantetheinyl Transferase Homolog Sfp on Streptococcus mutans Extracellular Membrane Vesicle Biogenesis

Extracellular membrane vesicles (EMVs) are produced by many Gram-positive organisms, but information regarding vesiculogenesis is incomplete. We used single gene deletions to evaluate the impacts on Streptococcus mutans EMV biogenesis of Sortase A (SrtA), which affects S. mutans...

2021-10-06 | MTBLS1812 | MetaboLights
MetabolomicsA multi-omics approach reveals functions of Secretory Carrier-Associated Membrane Proteins in wood formation of Populus trees
Secretory Carrier-Associated Membrane Proteins (SCAMPs) are highly conserved proteins that are involved in membrane trafficking. An integrated approach was taken to elucidate function of SCAMPs in wood formation of Populus trees. The metabolomics analyses were performed in woody tissues of transgeni...
2018-01-08 | MTBLS478 | MetaboLights
MetabolomicsMetabolome and proteome changes in skeletal muscle and blood of pre-weaning calves fed leucine and threonine supplemented diets

In pre-weaning calves, both leucine and threonine play important roles in growth and muscle metabolism. In this study, metabolomics, proteomics and clinical chemistry were used to assess the effects of leucine and threonine supplementation added to milk replacer on 14 newborn Holstein male calves...

2020-02-20 | MTBLS1045 | MetaboLights
ProteomicsProfiling antibody responses to M. tuberculosis proteins in TB suspects’ sera using full-proteome microarrays.
Understanding the immune response to tuberculosis requires greater knowledge of humoral responses. To characterize antibody targets and the effect of disease parameters on target recognition, we developed a systems immunology approach that integrated detection of antibodies against the entire Mycob...
ORGANISM(S): Homo sapiens 
2010-07-13 | E-GEOD-19433 | biostudies-arrayexpress
ProteomicsMetal organic layers enabled cell surface engineering coupling biomembrane fusion for dynamic membrane proteome profiling
Systematically dissecting the highly dynamic and tightly communicating membrane proteome of living cells is essential for systems-level understanding of fundamental cellular processes and intricate relationship between membrane-bound organelles constructed through membrane traffic. While extensive e...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD044945 | Pride
Membrane protein enrichment metal organic layers proteome 
ProteomicsProfiling proteome-scale antibody responses to M. tuberculosis
This SuperSeries is composed of the following subset Series: GSE30721: Profiling proteome-scale antibody responses to M. tuberculosis proteins in sera of macaques infected with M. tuberculosis GSE30722: Profiling proteome-scale antibody responses to M. tuberculosis proteins in TB suspect's sera Refe...
ORGANISM(S): Homo sapiens 
2012-07-05 | E-GEOD-31368 | biostudies-arrayexpress
ProteomicsMembrane proteome of HEK293T cells
We developed a simple and effective method for “in-depth” membrane proteomics. We optimize the sample preparation for the maximum numbers of the identified membrane proteins using a human cell line, HEK293T, as a model. From this study, we demystified the merits of Urea wash methods for membrane enr...
ORGANISM(S): Homo Sapiens (human) 
2022-04-28 | PXD027473 | JPOST Repository
ProteomicsA Peptidisc-based Survey of the Plasma Membrane Proteome of a Mammalian Cell
The low abundance and hydrophobicity of plasma membrane proteome relative to soluble proteins makes it difficult to characterize. Here, we apply the peptidisc membrane mimetic to purify the cell membrane proteome. We used HeLa cells as a reference to establish the method, and compared the plasma mem...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD039990 | Pride
Plasma membrane proteome Peptidisc 
ProteomicsMembrane proteome of renal BBMVs from the mouse model
In order to demonstrate the efficacy of the membrane enrichment method by Urea Wash in the animal model. Kidneys were dissected from 8 weeks old male mice of inbred strain C57BL/6J and flash-frozen in liquid nitrogen followed by storage at -80 °C. Brush border membrane vesicles (BBMV) were prepared...
ORGANISM(S): Mus Musculus (mouse) 
2022-04-28 | PXD027474 | JPOST Repository
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