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ProteomicsA Peptidisc-based Survey of the Plasma Membrane Proteome of a Mammalian Cell
The low abundance and hydrophobicity of plasma membrane proteome relative to soluble proteins makes it difficult to characterize. Here, we apply the peptidisc membrane mimetic to purify the cell membrane proteome. We used HeLa cells as a reference to establish the method, and compared the plasma mem...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD039990 | Pride
Plasma membrane proteome Peptidisc 
ProteomicsA Peptidisc-based Survey of the Plasma Membrane Proteome of a Mammalian Cell
The low abundance and hydrophobicity of plasma membrane proteome relative to soluble proteins makes it difficult to characterize. Here, we apply the peptidisc membrane mimetic to purify the cell membrane proteome. We used HeLa cells as a reference to establish the method, and compared the plasma mem...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD041913 | Pride
Hela Peptidiscs Membrane proteins Hpsc Panc-1 cells Lc-ms/ms 
UnknownThe Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution.
Not available
S-EPMC6093710 | biostudies-literature
UnknownA Peptidisc-Based Survey of the Plasma Membrane Proteome of a Mammalian Cell.
Not available
S-EPMC10416069 | biostudies-literature
UnknownProfiling the Escherichia coli membrane protein interactome captured in Peptidisc libraries.
Not available
S-EPMC6697469 | biostudies-literature
ProteomicsDevelopment of a Method Combining Peptidiscs and Proteomics to Identify, Stabilize and Purify a Detergent-Sensitive Membrane Protein Assembly
The peptidisc membrane mimetic enables global reconstitution of the bacterial membrane proteome into water-soluble detergent-free particles, termed peptidisc libraries. We present here a method that combines peptidisc libraries and chromosomal-level gene tagging technology with affinity purification...
ORGANISM(S): Escherichia coli 
2022-06-09 | PXD032315 | Pride
Peptidisc  membrane proteome  sec translocon  membrane protein complexes 
UnknownCharacterization of membrane protein interactions by peptidisc-mediated mass photometry.
Not available
S-EPMC10831248 | biostudies-literature
ProteomicsA peptidisc-based AP/MS approach to explore the interactome of the bacterial Sec translocon
We recently presented the peptidisc membrane mimetic for the global reconstitution of membrane proteomes into water-soluble detergent-free particles, termed peptidisc library. We here develop a method that combines the peptidisc library with affinity pulldown and mass spectrometry (AP/MS) to explore...
ORGANISM(S): Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911) 
2023-03-22 | PXD018866 | Pride
Mass spectrometry Affinity tagging Sec translocon Protein translocation Peptidisc Protein complex analysis Membrane mimetics Membrane protein complexes Protein-protein interactions Affinity purification/mass spectrometry (ap/ms) 
ProteomicsFunctionalized Peptidisc Scaffolds Enable Affinity Purification of the Membrane Proteome
We reconstituted the total E. coli membrane proteome into His-tagged peptidiscs. We then performed an affinity purification to enrich the bona fide membrane proteome away from soluble contaminants which co-sediment with cellular membranes after cell lysis. As a case study, we employ this method to s...
ORGANISM(S): Escherichia coli 
2022-04-11 | PXD017242 | Pride
Membrane protein reconstitution E. coli Peptidisc Membrane interactome 
ProteomicsProfiling the Liver Membrane Proteome in the Context of Liver Disease
Alcohol consumption and high-fat diets often coincide in Western society, exerting negative synergistic effects on the liver. While many studies have demonstrated the impact of ALD and NAFLD on protein expression, none have offered a comprehensive view of the dysregulation at the level of the membra...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-06 | PXD050825 | Pride
Plasma membrane Membrane mimetics Mass spectrometry Integral membrane proteins Proteome Surface markers Liver fibrosis Nafld 
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