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UnknownNative mass spectrometry of membrane proteins reconstituted in peptidiscs.
Not available
S-EPMC12560048 | biostudies-literature
UnknownNew approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteins.
Not available
S-EPMC7053995 | biostudies-literature
ProteomicsDevelopment of a Method Combining Peptidiscs and Proteomics to Identify, Stabilize and Purify a Detergent-Sensitive Membrane Protein Assembly
The peptidisc membrane mimetic enables global reconstitution of the bacterial membrane proteome into water-soluble detergent-free particles, termed peptidisc libraries. We present here a method that combines peptidisc libraries and chromosomal-level gene tagging technology with affinity purification...
ORGANISM(S): Escherichia coli 
2022-06-09 | PXD032315 | Pride
Peptidisc  membrane proteome  sec translocon  membrane protein complexes 
UnknownCryo-EM structure of the trehalose monomycolate transporter, MmpL3, reconstituted into peptidiscs.
Not available
S-EPMC10682824 | biostudies-literature
UnknownStructures of channelrhodopsin paralogs in peptidiscs explain their contrasting K+ and Na+ selectivities.
Not available
S-EPMC10359266 | biostudies-literature
ProteomicsA Simple Method to Isolate and Characterize a Bacterial Outer Membrane Proteome by Bottom-Up Mass Spectrometry
The outer membrane of gram-negative bacteria plays a critical role in protecting the cell against external stressors, including antibiotics. Given its importance to the resistance mechanisms, the outer membrane is a prime target for antimicrobial drug discovery. To facilitate discovery efforts, howe...
ORGANISM(S): Escherichia coli 
2023-03-11 | PXD036749 | Pride
Peptidiscs Outer membrane proteome Antibiotic resistance E. coli 
ProteomicsFunctionalized Peptidisc Scaffolds Enable Affinity Purification of the Membrane Proteome
We reconstituted the total E. coli membrane proteome into His-tagged peptidiscs. We then performed an affinity purification to enrich the bona fide membrane proteome away from soluble contaminants which co-sediment with cellular membranes after cell lysis. As a case study, we employ this method to s...
ORGANISM(S): Escherichia coli 
2022-04-11 | PXD017242 | Pride
Membrane protein reconstitution E. coli Peptidisc Membrane interactome 
ProteomicsA peptidisc-based AP/MS approach to explore the interactome of the bacterial Sec translocon
We recently presented the peptidisc membrane mimetic for the global reconstitution of membrane proteomes into water-soluble detergent-free particles, termed peptidisc library. We here develop a method that combines the peptidisc library with affinity pulldown and mass spectrometry (AP/MS) to explore...
ORGANISM(S): Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911) 
2023-03-22 | PXD018866 | Pride
Mass spectrometry Affinity tagging Sec translocon Protein translocation Peptidisc Protein complex analysis Membrane mimetics Membrane protein complexes Protein-protein interactions Affinity purification/mass spectrometry (ap/ms) 
UnknownCapture of endogenous lipids in peptidiscs and effect on protein stability and activity.
Not available
S-EPMC10993126 | biostudies-literature
UnknownCharacterization of membrane protein interactions by peptidisc-mediated mass photometry.
Not available
S-EPMC10831248 | biostudies-literature
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