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ProteomicsProximity Profiling of Chloroplast–Endoplasmic Reticulum Membrane Contact Sites in Nicotiana benthamiana Using Split-TurboID During Viral Infection
We fused the N-terminus of TurboID to the chloroplast outer membrane protein OEP7 and the C-terminus to the endoplasmic reticulum membrane protein RTNLB2. When the chloroplast and endoplasmic reticulum come into close proximity to form membrane contact sites (MCSs), the split-TurboID is reconstitute...
ORGANISM(S): Nicotiana Benthamiana 
PXD064967 |
MetabolomicsThe Toxoplasma monocarboxylate transporters are involved in the metabolism within the apicoplast and are linked to parasite survival

The apicoplast is a four-membrane plastid found in the apicomplexans, which harbors biosynthesis and organelle housekeeping activities in the matrix. However, the mechanism driving the flux of metabolites, in and out, remains unknown. Here we used TurboID and genome engineering to identify apicop...

2024-01-15 | MTBLS7060 | MetaboLights
ProteomicsThe interactome of the UapA transporter reveals putative new players in anterograde membrane cargo trafficking: ΔartA: UapA-TurboID
Neosynthesized plasma membrane (PM) proteins co-translationally translocate to the ER, concentrate at regions called ER-exit sites (ERes) and pack into COPII secretory vesicles which are sorted to the early-Golgi through membrane fusion. Following Golgi maturation, membrane cargoes reach the late-Go...
ORGANISM(S): Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) 
2023-11-21 | PXD043944 | Pride
Er-exit  copii  copi  traffic  fungi  a. nidulans Golgi-bypass  uapa  proximity dependent biotinylation  proteomics 
ProteomicsThe interactome of the UapA transporter reveals putative new players in anterograde membrane cargo trafficking: UapA-DYDY TurboID
Neosynthesized plasma membrane (PM) proteins co-translationally translocate to the ER, concentrate at regions called ER-exit sites (ERes) and pack into COPII secretory vesicles which are sorted to the early-Golgi through membrane fusion. Following Golgi maturation, membrane cargoes reach the late-Go...
ORGANISM(S): Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) 
2023-11-21 | PXD043941 | Pride
Er-exit  copii  copi  traffic  fungi  a. nidulans Golgi-bypass  uapa  proximity dependent biotinylation  proteomics 
ProteomicsThiol-cleavable Biotin for Chemical and Enzymatic Biotinylation and its Application to Mitochondrial TurboID Proteomics
Protein biotinylation via chemical or enzymatic reactions is often coupled with streptavidin-based enrichment and on-beads digestion in numerous biological applications. However, the popular on-beads digestion method faces major challenges of streptavidin contamination, the lost information of bioti...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-04-21 | MSV000087256 | MassIVE
ProteomicsTurboID-based proximity labeling identifies reticulon-like protein B2 as a pro-viral factor coopted for the biogenesis of viral replication organelles in plants
Although intensive studies have been performed to identify and characterize host factors involved in viral replication complex formation, the high dynamism of this process and the insoluble properties of the complex make it difficult to be analyzed comprehensively using traditional affinity purifica...
ORGANISM(S): Nicotiana Benthamiana 
2023-02-12 | PXD040073 |
ProteomicsRapid proximity biotinylation of the Plasmodium falciparum exported protein, SBP1
The deadly human malaria-causing parasite, Plasmodium falciparum relies on its capacity to completely remodel its host red blood cell (RBC) through the export of several hundred parasite proteins across several membranes to the RBC.We fused the exported membrane protein, skeleton binding protein 1 (...
ORGANISM(S): Plasmodium Falciparum Nf54 (ncbitaxon:5843) 
2022-06-27 | MSV000089743 | MassIVE
ProteomicsProximity labeling on phosphatidic acid-enriched membranes generated via optogenetic membrane editing (set2)
Cellular lipid metabolism is subject to strong homeostatic regulation, but players involved in and mechanisms underlying these pathways remain mostly uncharacterized. Here, we develop and exploit a “Feeding-Fishing” approach coupling membrane editing using optogenetic lipid-modifying enzymes (feedin...
ORGANISM(S): Homo sapiens (Human) 
2026-02-23 | PXD070270 | Pride
Feeding-fishing Membrane-turboid Optopld Membrane editing Phospholipase d Phosphatidic acid 
ProteomicsProximity labeling on phosphatidic acid-enriched membranes generated via optogenetic membrane editing
Cellular lipid metabolism is subject to strong homeostatic regulation, but players involved in and mechanisms underlying these pathways remain mostly uncharacterized. Here, we develop and exploit a “Feeding-Fishing” approach coupling membrane editing using optogenetic lipid-modifying enzymes (feedin...
ORGANISM(S): Homo sapiens (Human) 
2026-02-23 | PXD070378 | Pride
Feeding-fishing Membrane-turboid Optopld Membrane editing Phospholipase d Phosphatidic acid 
UnknownSplit-TurboID enables contact-dependent proximity labeling in cells.
Not available
S-EPMC7275672 | biostudies-literature
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