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ProteomicsMammalian MemPrep establishes the lipid composition ER membranes in HEK293T cells
Mammalian MemPrep is an approach that enables the isolation of ER membranes from HEK293T cells at a purity suitable for quantitative proteomics and lipidomics. Using cleavable, epitope‑tagged variants of SEC61β and REEP5, which preferentially localize to ER sheets and tubules, respectively, we isola...
ORGANISM(S): Homo sapiens (Human) 
2026-09-03 | PXD075836 | Pride
Er membrane composition Immunoisolation Mammalian cells Memprep Differential centrifugation 
ProteomicsA new technology for isolating organellar membranes provides fingerprints of lipid bilayer stress
Biological membranes have a stunning ability to adapt their composition in response to physiological stress and metabolic challenges. Little is known how such perturbations affect individual organelles in eukaryotic cells. Pioneering work provided insights into the subcellular distribution of lipids...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-03-05 | PXD048553 | Pride
Organelle isolation Proteomics Upr Lipid bilayer stress Lipidomics Membrane 
UnknownMemPrep, a new technology for isolating organellar membranes provides fingerprints of lipid bilayer stress
Not available
S-SCDT-10_1038-S44318-024-00063-Y | biostudies-other
UnknownMammalian MemPrep establishes the lipid composition of ER membranes in HEK293T cells
Not available
S-SCDT-10_1038-S44319-026-00952-9 | biostudies-other
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