We extracted mitochondria from Hela cells which were treated with CCCP or DMSO for 2 h. The samples were digested by FAST and peptides were used DIA to analysis.
We determined the binding and ubiquitination of HIF1a by an E3 ligase TRIM1. IP experiments were performed from SW480 cells and Ms files were acquired on Q Exactive mass spectrometer. We analysed the differences of protein abundance, comparing the normalized intensity on distinct proteins from diffe...
