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TranscriptomicsTranscription profiling of wild type G. sulfurreducens DL1 strain and mutant delta-sfrAB::kan, acetate-adapted strain using fumarate as an electron acceptor
1. Summary: SfrAB, the soluble ferric reductase of Geobacter sulfurreducens, is a two subunit complex that can catalyze the NADPH-dependent reduction of chelated Fe(III) and is encoded in a two gene cluster in which the sfrB gene (GSU_0510) is 126 bp upstream of the sfrA gene (GSU_0509)[Kaufmann an...
ORGANISM(S): Geobacter sulfurreducens 
2008-01-03 | E-TIGR-130 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of G sulfurreducens GGS nitrogen fixation
G. sulfurreducens (ATCC #51573) was obtained from the laboratory culture collection of Dr. Derek Lovley. Cells were grown under strict anaerobic conditions at 30 °C in chemostats, as previously described (for more information see Esteve-Núñez, A., M. M. Rothermich, M. Sharma, and D. R. Lovley. 2004....
ORGANISM(S): Geobacter sulfurreducens 
2005-01-01 | E-TIGR-82 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Geobacter sulfurreducens wild type and pilR mutants with acetate as the electron donor and ferric citrate as the electron acceptor
G. sulfurreducens wild type and pilR mutant (GSU1495) were grown in chemostats for RNA extraction used for microarray analysis and qRT-PCR. The electron donor (acetate 5mM) was limiting at a dilution rate of 0.05 h-1 and ferric citrate(55mM) was used as the electron acceptor at 30C, as previously de...
ORGANISM(S): Geobacter sulfurreducens 
2007-11-10 | E-JCVI-1 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of wild type G. sulfurreducens DL1 strain and mutant DLCN16 (delta-rpoS::Km) using fumarate as an electron acceptor
Wild type G. sulfurreducens DL1 strain (see Caccavo, F., Jr., D. J. Lonergan, D. R. Lovley, M. Davis, J. F. Stolz, and M. J. McInerney. 1994. Geobacter sulfurreducens sp. nov., a hydrogen- and acetate-oxidizing dissimilatory metal-reducing microorganism. Appl Environ Microbiol 60:3752-9. see also Co...
ORGANISM(S): Geobacter sulfurreducens 
2005-01-01 | E-TIGR-128 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of wild type G. sulfurreducens DL1 strain and mutant DLCN16 (delta-rpoS::Km) using ferric citrate as an electron acceptor
Wild type G. sulfurreducens DL1 strain (see Caccavo, F., Jr., D. J. Lonergan, D. R. Lovley, M. Davis, J. F. Stolz, and M. J. McInerney. 1994. Geobacter sulfurreducens sp. nov., a hydrogen- and acetate-oxidizing dissimilatory metal-reducing microorganism. Appl Environ Microbiol 60:3752-9. see also Co...
ORGANISM(S): Geobacter sulfurreducens 
2005-01-01 | E-TIGR-123 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of G sulfurreducens GGS Fe(III) vs fumarate
G. sulfurreducens (ATCC #51573) was obtained from the laboratory culture collection of Dr. Derek Lovley. Cells were grown under strict anaerobic conditions at 30 °C in chemostats, (see Esteve-Núñez, A., M. M. Rothermich, M. Sharma, and D. R. Lovley. 2004. Growth of Geobacter sulfurreducens under nut...
ORGANISM(S): Geobacter sulfurreducens 
2005-01-01 | E-TIGR-81 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Geobacter sulfurreducens grown in batch culture with either ferric citrate or graphite anodes as the electron acceptor
G. sulfurreducens (ATCC #51573) was obtained from the laboratory culture collection of Dr. Derek Lovley. Cells were grown under strict anaerobic conditions at 23 °C in batch culture. Both control and experimental populations were grown with 10mM acetate as the electron donor while Fe(III) as 55 mM f...
ORGANISM(S): Geobacter sulfurreducens 
2007-07-02 | E-TIGR-5001 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of wild type G. sulfurreducens DL1 strain and mutant RelA (relGsu::kan) using fumarate as an electron acceptor
This experiment tests the gene expression of the RelA mutant (ORF03691/GSU2236) versus wild type G. sulfurreducens during log phase growth. Chemostat culture, Acetate-limiting electron donor, Fumarate electron acceptor.
ORGANISM(S): Geobacter sulfurreducens 
2007-02-15 | E-TIGR-200 | biostudies-arrayexpress
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