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Significant technological advances in both affinity chromatography and mass spectrometry have facilitated the identification of peptides associated with the major histocompatibility complex class I (MHC I) molecules, and enabled a greater understanding of the dynamic nature of the immunopeptidome of...
ORGANISM(S): Homo sapiens (Human) 
2018-03-09 | PXD007935 | Pride
Hardy’s Fraction D bone marrow pre-B cells and splenic follicular B cells were purified by flow cytometry according to MHC II expression from wild-type and ItgaxcreH2-Ab1c MHC II conditional mice and transcriptionally profiled by RNA-seq. Duplicate or triplicate samples for each genotype and level o...
ORGANISM(S): Mus musculus 
F6 murine leukaemic cells were transfected either with MHC II or with GFP and transcriptionally profiled by RNA-seq. Triplicate samples for each genotype were sequenced. This is part of ongoing investigation of a possible cell-intrinsic role of MHC II B cells.
ORGANISM(S): Mus musculus 
Indian rhesus macaques are arguably the most reliable animal models in AIDS research. In this species the MHC class I allele Mamu-B*08, among others, is associated with elite control of SIV replication. A similar scenario is observed in humans where the expression of HLA-B*27 or HLA-B*57 has been li...
ORGANISM(S): Homo sapiens (Human) 
2016-10-07 | PXD004964 | Pride
Effect of MHC II expression in pro-B cells: Pre-B cells and follicular B cells from wild-type and MHC II conditional mice
Primary murine B cells were stimulated with IL2, IL5, LPS to induce B cell differentiation for 24 hours. RNA was extracted from control and differentiated B cell to compare gene expression. Murine B cells (CD43-) were purfied from 5 weeks old mice splenocytes. And purified B cells were stimulated wi...
ORGANISM(S): Mus musculus 
Antigen presentation by B cells enables epitope spreading across an MHC barrier
Effect of MHC II expression in pro-B cells: F6 leukaemic cell line
An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
Marginal Zone B Cells Trogocytose from Dendritic Cells and Present to T cells MHC II–C3 complexes
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