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ProteomicsMass spectrometry identification of PPARγ methylation sites
Purified recombinant PPARγ-Flag and PRMT4-His were used for in vitro methyltransferase assay.We separated purified methylated PPARγ proteins by SDS-PAGE, visualized them by staining with Coomassie blue, protein lanes were cut into pieces about 1 mm3, and followed further destained.
ORGANISM(S): Homo Sapiens 
2022-06-27 | PXD034929 |
UnknownOverexpression of rice aquaporin OsPIP1;2 improves yield by enhancing mesophyll CO2 conductance and phloem sucrose transport.
Not available
S-EPMC6322580 | biostudies-literature
ProteomicsClinicopathological Observation and Initial Exploration of Characteristic Biomarkers in Apocrine Breast Carcinoma
Perform proteomic profiling comparing triple-negative apocrine carcinoma (TNAC) and luminal androgen receptor-type triple-negative breast cancer (LAR-TNBC) to elucidate their molecular distinctions and validate TNAC as a distinct entity separate from LAR-TNBC.
ORGANISM(S): Homo Sapiens 
2026-01-26 | PXD073549 |
ProteomicsRegulation of Enrofloxacin drug resistance in bovine Pasteurella multocida strains by quorum-sensing acyl-homoserine lactone signaling molecules
Pasteurella multocida (Pm) is an important pathogen causing respiratory disease in beef cattle, often forming mixed infections with Mycoplasma bovis and other pathogens, seriously jeopardizing the healthy development of the cattle industry. Fluoroquinolones and other broad-spectrum antimicrobials ar...
ORGANISM(S): Pasteurella Multocida Subsp. Multocida Str. Anand1_cattle 
2025-10-18 | PXD069645 |
ProteomicsTargeting Lipid raft-related stomatin strategy for challenging osteoporosis
Mouse bone marrow macrophages, with or without the addition of RANKL, are being investigated for their interactions with proteins that associate with the stomatin protein.
ORGANISM(S): Mus Musculus 
2025-03-13 | PXD061779 |
ProteomicsMS analysis of endogenously precipitated DDX20 protein complexes (immunoprecipitation (IP)–MS)
Protein lysates were derived from HEK293T cells and spermatogonial stem cells，and interacting proteins were “pulled-down” for analysis by gel electrophoresis and identification by mass spectrometry. An equal amount of non-specific IgG from rabbit serum (Thermos Fisher Scientific) was used as a negat...
ORGANISM(S): Homo Sapiens Mus Musculus 
2024-04-24 | PXD038171 |
ProteomicsProteomic analysis of the effects of DDX20 deletion in spermatogonia
To investigate the role of DDX20 in spermatogenesis, we generated Ddx20 flox/flox Ddx4-Cre mice to make a germ cell-specific Ddx20 knockout, and isolated spermatogonia from four-day-old mouse testes by THY1 magnetic beads. We then performed proteomic analysis using protein lysates obtained from THY1...
ORGANISM(S): Mus Musculus 
2024-04-24 | PXD038170 |
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