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Capitalizing on the massive increase in sample concentrations which are produced by extremely low elution volumes, nano-LC-ESI-MS/MS is currently the most sensitive analytical technology for the comprehensive characterization of complex protein samples. However, despite tremendous technological impr...
ORGANISM(S): Homo sapiens (Human) 
2019-10-23 | PXD014124 | Pride
In the light of the ongoing single-cell revolution, scientific disciplines are combining forces with the ultimate goal to retrieve as much relevant data as possible from trace amounts of biological material. For single cell proteomics, this implies optimizing the entire workflow from initial cell is...
ORGANISM(S): Homo sapiens (Human) 
2021-06-28 | PXD024017 | Pride
Reversed-phase chromatography is the most common technique for separation of tryptic peptides. In this manuscript, we describe the optimization of sample loading and separation parameters for a novel micro machined column and provide a detailed description on the performance and reproducibility of t...
ORGANISM(S): Homo sapiens (Human) 
2019-07-18 | PXD013158 | Pride
Nanoflow liquid chromatography capable of delivering consistent and reliable results across extensive sample sets is essential for the advancement of mass spectrometry-based proteomics. Micro-Pillar Array Columns (μPACs) represent a significant breakthrough, offering durability and performance stabi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2025-11-24 | PXD067649 | Pride
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