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To understand the basic biological properties of supporting cells of the mammalian inner ear, we examined the transcriptome of adult pillar and Deiters’ cells. Morphologically distinct pillar and Deiters’ cells were isolated from the organ of Corti from adult CBA/J mice. One thousand pillar and Deit...
ORGANISM(S): Mus musculus 
2018-08-03 | GSE111347 | GEO
Capitalizing on the massive increase in sample concentrations which are produced by extremely low elution volumes, nano-LC-ESI-MS/MS is currently the most sensitive analytical technology for the comprehensive characterization of complex protein samples. However, despite tremendous technological impr...
ORGANISM(S): Homo sapiens (Human) 
2019-10-23 | PXD014124 | Pride
Reversed-phase chromatography is the most common technique for separation of tryptic peptides. In this manuscript, we describe the optimization of sample loading and separation parameters for a novel micro machined column and provide a detailed description on the performance and reproducibility of t...
ORGANISM(S): Homo sapiens (Human) 
2019-07-18 | PXD013158 | Pride
RNA-sequencing of Adult Mouse Pillar and Deiters’ Supporting Cells of the Organ of Corti
Dendrogyra cylindrus (Pillar coral) whole genome sequencing - TCRF, ORG.one, and Darwin Plus project
The project establishes an easy-to-follow protocol to set up bottom-up proteomics experiments using state-of-the-art pillar-array columns and mass spectrometry. The protocol touches upon key points of the method development, discusses the trade-off between proteome depth and analysis time and demons...
ORGANISM(S): Homo sapiens (Human) 
2026-01-13 | PXD069993 | Pride
In the light of the ongoing single-cell revolution, scientific disciplines are combining forces with the ultimate goal to retrieve as much relevant data as possible from trace amounts of biological material. For single cell proteomics, this implies optimizing the entire workflow from initial cell is...
ORGANISM(S): Homo sapiens (Human) 
2021-06-28 | PXD024017 | Pride
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