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ProteomicsSimple Strategy of Integrating the Micro-centrifugation-based Extraction and 4-Hexylphenylazosulfonate for the Preparation of Plant Microsomal Proteins for Bottom-Up Proteomics
The preparation of high-quality microsomal proteins (MPs) is of critical importance to high-throughput microsomal proteomics. The isolation of MPs for proteomic analysis has to date relied on Ultracentrifugation-based methods (UBMs), however, these methods are labor-intensive, time-consuming, and un...
ORGANISM(S): Oryza sativa subsp. japonica (Rice) 
2021-09-29 | PXD025132 | Pride
Rice leaves Perseus Label-free quantitative proteomics Ultracentrifugation Micro-centrifugation Azo Maxquant Microsomal membrane extraction Plasma membrane Sds 
TranscriptomicsTranscriptomic analysis of chlorhexidine shock in Acinetobacter baumannii
In this study the transcriptomic changes occurring in A. baumannii ATCC 17978 in response to shock treament using the biocide chlorhexdine were determined. A. baumannii ATCC 17978 cells were grown at 37°C with shaking (200rpm) in 35mL cultures in MH broth to OD600=0.75, at which time they were spli...
ORGANISM(S): Acinetobacter baumannii 
2013-12-03 | E-GEOD-51525 | biostudies-arrayexpress
TranscriptomicsCLN3 patients with differing progression of the disease
Mutations in the CLN3 gene lead to juvenile neuronal ceroid lipofuscinosis, a pediatric neurodegenerative disorder characterized by visual loss, epilepsy and psychomotor deterioration. Although most CLN3 patients carry the same 1 kb deletion in the CLN3 gene, their disease phenotype can be variable....
ORGANISM(S): Homo sapiens 
2011-06-08 | E-GEOD-22225 | biostudies-arrayexpress
UnknownA low-cost, open-source centrifuge adaptor for separating large volume clinical blood samples.
Not available
S-EPMC9269434 | biostudies-literature
OtherSlf1p and Sro9p affinity isolations
To identify RNAs specifically associated with Slf1p and Sro9p, cells expressing TAP-tagged Slf1, Sro9 or Fpr1 (negative control) were grown to mid-log phase in synthetic complete medium and harvested by centrifugation. RNA affinity isolations were essentially performed as described (Gerber et al. 20...
ORGANISM(S): Saccharomyces cerevisiae 
2012-02-08 | GSE27310 | GEO
GenomicsExtracellular vesicles secreted from human myotubes of T2D and NGT donors
Type 2 diabetes (T2D) is associated with increased morbidity, mortality, and substantial healthcare costs. Peripheral insulin resistance, involving interconnected dysregulation of multiple organs, is considered a major driver of T2D. Extracellular vesicles (EVs) are suggested as mediators of this dy...
ORGANISM(S): Homo sapiens synthetic construct 
2025-11-21 | GSE307642 | GEO
MetabolomicsThe dynamic changes and mechanism of no reflow injury after myocardial ischemia-reperfusion
This study aims to investigate the dynamic changes and correlation of no-reflow (NR) injury following myocardial ischemia and reperfusion, and to explore the underlying mechanism of NR injury through transcriptomic and metabolomic analysis. Methods: Healthy male SD rats were divided into two batches...
2026-08-25 | MTBLS15452 | MetaboLights
TranscriptomicsTranscriptomic analysis of chlorhexidine shock in Acinetobacter baumannii
In this study the transcriptomic changes occurring in A. baumannii ATCC 17978 in response to shock treament using the biocide chlorhexdine were determined. A. baumannii ATCC 17978 cells were grown at 37°C with shaking (200rpm) in 35mL cultures in MH broth to OD600=0.75, at which time they were split...
ORGANISM(S): Acinetobacter baumannii 
2013-12-03 | GSE51525 | GEO
TranscriptomicsSf1+ mouse adrenocortical cells vs. Wnt-responsive mouse adrenocortical cells
We sought to determine which gene transcripts are enriched in Wnt-responsive adrenocortical mouse cells compared to the entire adrenocortical mouse cell population in vivo. To this end, we employed transgenic reporter mice that label Wnt-responsive cells with GFP expression (TCF/Lef:H2B-GFP mice) or...
ORGANISM(S): Mus musculus 
2014-07-11 | E-GEOD-53981 | biostudies-arrayexpress
UnknownTotal organic carbon (TOC): a simple tool for assessing micro(nano)plastics and nanocellulose recovery during size-based fractionation.
Not available
S-EPMC12103321 | biostudies-literature
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