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ProteomicsMicro-flow LC-MS/MS Based Single Shot Proteomics
In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run i...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Mus musculus (Mouse) Homo sapiens (Human) 
2021-07-01 | PXD023587 | Pride
Single shot proteomics Micro-flow lc 1.0 mm id column 
ProteomicsComparision of Nano and Micro-flow LC-MS/MS Based Single Shot Proteomics
In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run i...
ORGANISM(S): Homo sapiens (Human) 
2021-07-01 | PXD024169 | Pride
Single shot proteomics Nano-flow lc Micro-flow lc 
ProteomicsRobust micro-flow LC-MS/MS for proteome analysis – 38,000 runs and counting
Micro-flow liquid chromatography tandem mass spectrometry (µLC–MS/MS) is increasingly becoming an alternative to nano-LC–MS/MS for the analysis of proteomes. We have recently demonstrated the potential of a µLC–MS/MS system operating with a 1 mm i.d. × 150 mm column and at a flow rate of 50 µl/min f...
ORGANISM(S): Homo sapiens (Human) 
2021-02-22 | PXD023650 | Pride
Micro-flow lc Plasma Proteomics Urine 1.0 mm id column 
ProteomicsHuman milk protein components assessed by proteome analysis on a fast and robust micro-flow LC‒MS/MS system
Human milk is considered the best food for infants and young children, and an in-depth exploration of its molecular composition could provide a scientific basis for the development and production of substitutes. Therefore, we developed a micro-flow liquid chromatography tandem mass spectrometry (μLC...
ORGANISM(S): Homo Sapiens 
2026-02-10 | PXD074321 |
UnknownRobust, reproducible and quantitative analysis of thousands of proteomes by micro-flow LC-MS/MS.
Not available
S-EPMC6952431 | biostudies-literature
UnknownCombination of automated sample preparation and micro-flow LC-MS for high-throughput plasma proteomics.
Not available
S-EPMC9824974 | biostudies-literature
MetabolomicsCE-MS Metabolomic and LC-MS Proteomic Analyses of Breast Cancer Exosomes Reveal Alterations in Purine and Carnitine Metabolism

A nano sheath-flow capillary electrophoresis mass spectrometry (CE-MS) system with electrospray ionization was used to profile cationic metabolite cargo in exosomes secreted by non-tumorigenic MCF-10A and tumorigenic MDA-MB-231 breast epithelial cells. CE-MS-based metabolomics revealed increased ...

2024-11-14 | MTBLS11190 | MetaboLights
ProteomicsRobust micro-flow LC-MS/MS for proteome analysis – 38,000 runs and counting
Micro-flow liquid chromatography tandem mass spectrometry (µLC–MS/MS) is increasingly becoming an alternative to nano-LC–MS/MS for the analysis of proteomes. We have recently demonstrated the potential of a µLC–MS/MS system operating with a 1 mm i.d.?×?150?mm column and at a flow rate of 50?µl/min f...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-06-27 | MSV000098370 | MassIVE
UnknownSystematic Investigation of LC Miniaturization to Increase Sensitivity in Wide-Target LC-MS-Based Trace Bioanalysis of Small Molecules.
Not available
S-EPMC9340153 | biostudies-literature
UnknownA Micro-Flow Liquid Chromatography-Mass Spectrometry Method for the Quantification of Oxylipins in Volume-Limited Human Plasma.
Not available
S-EPMC11952279 | biostudies-literature
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