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ProteomicsMicro-scale RPLC-CZE-MS/MS for deep and highly sensitive bottom-up proteomics
Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has been well recognized for bottom-up proteomics. It has approached 4000-8000 protein identifications (IDs) from a human cell line, mouse brains or Xenopus embryos via coupling with liquid chromatography (LC) prefractionation. Howe...
ORGANISM(S): Homo sapiens (Human) 
2018-08-16 | PXD009991 | Pride
Micro-scale rplc Cze-ms/ms 
ProteomicsTime-resolved profiling of mRNA-protein interaction through mRNA life cycle
From synthesis to decay, mRNA changes its protein partners, yet previous studies were limited in that they profiled only the mixed pools of RNA-binding proteins (RBPs) without temporal resolution. Here, we provide time-resolved profiles of human mRNA interactome by combining pulse metabolic labeling...
ORGANISM(S): Homo sapiens (Human) 
2024-02-23 | PXD039054 | Pride
Mrna-interactome Hela Temporal Rna-interactome Mrna Rna Stage Rbp Age Chronology 
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