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The inactive X chromosome (Xi) serves as a model to understand gene silencing on a global scale. Here, we perform identification of direct RNA interacting proteins? (iDRiP) to isolate a comprehensive protein interactome for Xist, an RNA required for Xi silencing. We discover multiple classes of inte...
ORGANISM(S): Mus musculus 
LncPHx2 RNA-interactome study realed that LncPHx2 is associated with multiple RNAs Biotinylated DNA probes targeting LncPHx2 were used to precipitate endogenous LncPHx2 and associated RNAs from Hepa 1-6 cells. The associated RNAs were sequenced and 415 unique transcripts were identified.
ORGANISM(S): Mus musculus 
CTCF is a master regulator that plays a role in genome architecture and gene expression. A key aspect of CTCF’s mechanism involves bringing together distant genetic elements for intra- and inter-chromosomal interactions. Evidence from epigenetic processes, such as X-chromosome inactivation (XCI), su...
ORGANISM(S): Mus musculus 
Formaldehyde crosslinking based profiling of RNA interactome in the mosue tissue, liver.
ORGANISM(S): Mus musculus (Mouse) 
2020-11-25 | PXD022023 | Pride
RNA-binding proteins (RPBs) are deeply involved in many fundamental cellular processes in bacteria and are vital for their survival. Despite this, few studies have so far been dedicated to globally identifying bacterial RBPs. We have adapted the RNA interactome capture (RIC) technique, originally de...
ORGANISM(S): Escherichia coli 
2023-03-30 | PXD032939 | Pride
Populations of small eukaryotic RNAs, in addition to relatively well recognized molecules (such as miRNAs or siRNAs), also contain fragments derived from all classes of constitutively expressed non-coding RNAs. It has been recently demonstrated that the formation and accumulation of RNA fragments (R...
ORGANISM(S): Homo sapiens (Human) 
2018-10-24 | PXD010075 | Pride
SARS-CoV-2 is an RNA virus whose success as a pathogen relies on its ability to repurpose host RNA-binding proteins (RBPs) to form its own RNA interactome. Here, we developed and applied a robust ribonucleoprotein capture protocol to uncover the SARS-CoV-2 RNA interactome. We report 109 host factors...
ORGANISM(S): Homo sapiens (Human) Chlorocebus sabaeus 
2021-04-28 | PXD024808 | Pride
Comprehensive architecture of the bacterial RNA Interactome
ChIP-seq to investigate the phase separation-dependent FUS RNA interactome
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