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TranscriptomicsTranscription profiling of cod to determine whether gene expression profiles would reflect environmental levels and composition of pollutants in two saltwater recipients, Sarfjorden and Store Lungegardsvann
In the present study we aimed to develop a custom made cDNA microarray, the CodStress array, for Atlantic cod to be used as a diagnostic tool in environmental monitoring of polluted waters. We wanted to investigate if the gene expression profiles would reflect environmental levels and composition of...
ORGANISM(S): Gadus morhua 
2009-06-01 | E-BASE-11 | biostudies-arrayexpress
ProteomicsImproved protease digestion conditions for membrane protein detection
This work presents improved protease digestion conditions for membrane protein detection. Two improved conditions, 0.01% SDS or the combination of 10% MeOH and 0.01% RapiGest, were chosen and applied to proteins from Escherichia coli. The application of both improved conditions to a membrane protein...
ORGANISM(S): Escherichia coli 
2015-04-28 | PXD001824 | Pride
Proteomics Membrane protein E. coli 
GenomicsTOP1M-NM-1 functions on nucleosome density
DNA topoisomerases release supercoils in DNA introduced during replication or transcription. How DNA topoisomerases impact transcription in the context of eukaryotic chromatin is poorly understood. In this study, using a floral stem cell model in Arabidopsis, we uncovered a role of TOP1M-NM-1 in Pol...
ORGANISM(S): Arabidopsis thaliana 
2014-10-14 | E-GEOD-53950 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation profiling of human breast cancer cell lines and tissues to identify novel estrogen receptor-{alpha} binding sites and estradiol target genes
Estrogen receptor-{alpha} (ER{alpha}) and its ligand estradiol play critical roles in breast cancer growth and are important therapeutic targets for this disease. Using chromatin immunoprecipitation (ChIP)-on-chip, ligand-bound ER{alpha} was recently found to function as a master transcriptional reg...
ORGANISM(S): Homo sapiens 
2008-06-20 | E-GEOD-11506 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Atlantic cod exposed to alkylphenols and produced water
Female Atlantic cod (Gadus morhua)were devided in to five groups and oraly exposed to alkylphenols and produced water for 20 weeks. Differentially expressed genes were studied in liver samples using the CodStress array. The five groups were fed with feed-paste containing two different doses of the A...
ORGANISM(S): Gadus morhua 
2009-03-25 | E-TABM-676 | biostudies-arrayexpress
MetabolomicsABA accumulation confers cross-adaptation to saline-alkali and cold stresses in alfalfa

Combined saline-alkali and cold stresses severely constrain plant growth at middle and high latitudes. Plants have evolved cross-adaptation mechanisms wherein exposure to one stress enhances resistance to another. However, the specific mechanisms driving cross-adaptation between saline-alkali and...

2026-03-22 | MTBLS14108 | MetaboLights
ProteomicsInteraction with IP6K1 supports pyrophosphorylation of substrate proteins by the inositol pyrophosphate 5-IP7
HEK293T cells transiently transfected to express either SFB-tagged GFP or SFB-tagged IP6K1 were lysed in NETN buffer (20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40) containing protease and phosphatase inhibitor cocktails (Merck), at 4 degree C for 60 min. Cell debris were removed...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-06-20 | MSV000092218 | MassIVE
GenomicsMouse ES cells expressing catalytically inactive Ring1B display impaired Ring1B and H3K27me3 deposition.
ChIP-seq for H3K27me3 and Ring1B was performed in WT mESCs and mESCs containing catalytically inactive Ring1B (I53A mutant). Cells expressing catalytically inactive Ring1B maintain the spatial distribution of Ring1B and H3K27me3 but at reduced levels. These findings support the notion that PRC2 recr...
ORGANISM(S): Mus musculus 
2015-09-20 | E-GEOD-69955 | biostudies-arrayexpress
TranscriptomicsTime series of Methanococcus maripaludis MM901:del_MMP1100, a shift from a H2-excess and P-limiting condition to a H2-limited and P-excess condition in chemostats (growth rate held constant)
Methanogens catalyze the critical, methane-producing step (called methanogenesis) in the anaerobic decomposition of organic matter and have applications in carbon-neutral fuel production. Here, we present the first predictive model of global gene regulation of methanogenesis in a hydrogenotrophic me...
ORGANISM(S): Methanococcus maripaludis S2 
2013-09-16 | E-GEOD-42162 | biostudies-arrayexpress
TranscriptomicsTime series of Methanococcus maripaludis MM901:del_MMP1447, a shift from a H2-excess and P-limiting condition to a H2-limited and P-excess condition in chemostats (growth rate held constant)
Methanogens catalyze the critical, methane-producing step (called methanogenesis) in the anaerobic decomposition of organic matter and have applications in carbon-neutral fuel production. Here, we present the first predictive model of global gene regulation of methanogenesis in a hydrogenotrophic me...
ORGANISM(S): Methanococcus maripaludis S2 
2013-09-16 | E-GEOD-42164 | biostudies-arrayexpress
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