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Arabidopsis, PSS1, miniTurbo, proximity labeling, nonhost resistance, Phytophthora sojae, protein interactome, mass spectrometry
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Phytophthora sojae 
2026-06-11 | PXD065664 | Pride
The Arabidopsis PSS1 gene, which encodes a glycine-rich protein, plays a pivotal role in nonhost resistance to the soybean oomycete Phytophthora sojae and the fungal pathogen Fusarium virguliforme. To elucidate the molecular basis of PSS1-mediated immunity, we employed miniTurbo-based proximity labe...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Phytophthora sojae 
2026-06-11 | PXD065660 | Pride
TurboID-based proximity labeling of DMR6 and DLO1 identified several candidate interactors, including a putative F-box protein that may contribute to their SCF-dependent turnover.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Nicotiana benthamiana 
2026-03-06 | PXD063821 | Pride
we used proximity labeling of ASK1, a core SCF adaptor, to profile dynamic changes in SCF-associated networks during Pseudomonas syringae infection. This analysis revealed widespread remodeling of F-box proteins and immune-related regulators, including several previously uncharacterized components.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-03-05 | PXD063820 | Pride
Lysine acylation is a post-translational modification (PTM) conserved in all domains of life and is essential for regulating diverse biological processes. Traditional methods for investigating acylation rely on anti-acyl-lysine-antibodies, which are costly, time-consuming, and often exhibit variable...
ORGANISM(S): Pseudomonas aeruginosa (strain UCBPP-PA14) Staphylococcus aureus 
2026-06-11 | PXD072564 | Pride
Finely ground leaf tissue was used. Extraction was done in 8M urea, precipitated with acetone and methanol. Filter-assisted sample preparation (FASP) columns were used to further purify proteins. Purified proteins were reduced with TCEP, alkylated with iodoacetamide and digested into peptides with ...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-11-17 | MSV000086460 | MassIVE
Finely ground leaf tissue of wild-type columbia-0 ecotype was used. Protein extraction was performed in buffer-saturated phenol and high sucrose buffer. Extracted proteins were incubated with purified recombinant GST or GST-BIN2 kinase. Filter-assisted sample preparation (FASP) columns in prese...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-11-17 | MSV000086462 | MassIVE
Here we explore the feasibility of single-cell proteomics on plant samples. We focus on Arabidopsis thaliana, examining isolated single cells from the cortex and endodermis, which are two adjacent root cell-types derived from a common stem cell.
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-04-17 | MSV000094558 | MassIVE
The Microbe Associated Molecular Pattern flg22 is recognized in a FLAGELLIN-SENSITVE 2-dependent manner in root tip cells. Here, we show a rapid and massive change in protein abundance and phosphorylation state of the Arabidopsis root cell proteome in wildtype and a mutant deficient in heterotrimer...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2023-08-16 | MSV000092672 | MassIVE
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