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TranscriptomicsScf-GFP+ cells from the bone marrow and whole bone marrow microarray
The HSC niche factor SCF is required for HSC maintenance. Using an Scf-GFP knockin mouse, we have identified a perivascular cell type in the bone marrow expressing high level of Scf. To characterize the novel Scf-GFP+ cells from the bone marrow, we performed microarray analysis on these cells. Total...
ORGANISM(S): Mus musculus 
2012-02-01 | E-GEOD-33158 | biostudies-arrayexpress
TranscriptomicsSox17-transgenic hematopoietic stem cell microarray
The transcription factor SOX17 is expressed by fetal, but not adult hematoipoietic stem cells (HSCs), and is required for the maintenance of fetal and neonatal, but not adult, HSCs. In the current study we show that ectopic expression of Sox17 in adult HSCs and transiently reconstituting multipotent...
ORGANISM(S): Mus musculus 
2011-07-14 | E-GEOD-30445 | biostudies-arrayexpress
TranscriptomicsOncogenic Nras has a bimodal effect on hematopoietic stem cells promoting proliferation and self-renewal
Pre-leukemic mutations are thought to promote clonal expansion of hematopoietic stem cells (HSCs) by increasing self-renewal and competitiveness. However, mutations that increase HSC proliferation tend to reduce competitiveness and self-renewal potential, raising the question of how a mutant HSC can...
ORGANISM(S): Mus musculus 
2014-03-14 | E-GEOD-45194 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of brain and gut neurospheres of wild type and Bmi-1 knockout mice to reveal that Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation.
The cRNA derived from Brain and Gut neurospheres of wild type and BMI-1 knockout mice were hybridized to Affymetrix mouse ver 2 Chips A, B and C. In case of brain neurospheres, the cRNA were generated by one round RNA amplification (conventional method). In contrast, the cRNA of Gut neurospheres wer...
ORGANISM(S): Mus musculus 
2007-12-15 | E-GEOD-611 | biostudies-arrayexpress
TranscriptomicsRetroviral Sox17 over-expression adult hematopoietic stem/progenitor cells microarray
The transcription factor SOX17 is expressed by fetal, but not adult hematoipoietic stem cells (HSCs), and is required for the maintenance of fetal and neonatal, but not adult, HSCs. In the current study we show that ectopic expression of Sox17 in adult HSCs and transiently reconstituting multipotent...
ORGANISM(S): Mus musculus 
2011-07-14 | E-GEOD-30444 | biostudies-arrayexpress
TranscriptomicsPrdm16 newborn mouse (ventricular zone) VZ cells microarray
As Prdm16 deficiency reduces self-renewal potential and depletes neural stem cells in culture we decided to investigate the underlying molecular mechanisms of the neural stem cells depletion in the Prdm16 deficient animals. For the experiment we used Prdm16Gt(OST67423)Lex (Prdm16LacZ) genetrap mice ...
ORGANISM(S): Mus musculus 
2010-09-09 | E-GEOD-23406 | biostudies-arrayexpress
TranscriptomicsZinc finger nuclease knockouts of human ADP-glucokinase
Zinc finger nucleases (ZFN) are powerful tools for editing genes in cells. Here we use ZFNs to interrogate the biological function of human ADPGK, which encodes an ADP-dependent glucokinase (ADPGK), in tumour cell lines. The hypothesis tested is that ADPGK utilises ADP to phosphorylate glucose under...
ORGANISM(S): Homo sapiens 
2012-07-19 | E-GEOD-39497 | biostudies-arrayexpress
TranscriptomicsHistone deacetylase inhibition replicates aspects of the adaptive response to exercise and improves muscle metabolism and cardiac function in obesity.
Drugs used to treat obesity and type 2 diabetes have limited efficacy in directly normalising muscle metabolism. The discovery of molecules mediating exercise adaptations, and drugs that modulate their activity, is a potential strategy to address this therapeutic gap. Here we show that genetic impai...
ORGANISM(S): Mus musculus 
2015-01-28 | E-GEOD-54642 | biostudies-arrayexpress
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