S. cerevisiae expressing the wild-type C. albicans G33 ser-tRNACAG and the mutant T33 ser-tRNACAG that trigger genetic code ambiguity were compared against control yeast using two-color oligo arrays
This study integrated functional assessments of ram sperm (motility, viability, membrane and acrosome integrity, chromatin stability) with shotgun proteomics (LC-MS/MS, label-free) to identify molecular signatures associated with cryotolerance. Hair rams were classified into high- and low-freezabili...