Using H1299 wild-type cells and their MPND-knockout derivatives as research subjects, this project employs Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for metabolic labeling of the two cell groups. Combined with ubiquitinated peptide-specific enrichment and nano-liquid chromatogra...
he Mpr1/Pad1 N-terminal domain-containing protein (MPND) belongs to the JAMM family of deubiquitinases and is ubiquitously expressed across human tissues. However, its role in non-small cell lung cancer (NSCLC) remains unexplored. In this study, we demonstrate that MPND expression is frequently redu...
In this study, H1975 wild-type and MPND-knockout cells were used for SILAC-based quantitative ubiquitin proteomics. Ubiquitinated peptides were enriched and analyzed by nano-LC-MS/MS. MaxQuant was used for protein quantification and differential ubiquitination site screening. Bioinformatic analyses ...
