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TranscriptomicsKnockdown of SR protein family in P19 cells and RNA-seq of cytoplasmic and whole cell polyA+ RNA
Our data suggest that all SR proteins contribute to mRNA export via NXF1. To identify endogenous export targets we depleted all seven SR proteins individually from P19 WT cells prepared cytoplasmic fractions. We sequenced the cytoplasmic fraction and as a control whole celll RNA from the identical s...
ORGANISM(S): Mus musculus 
2016-03-07 | E-GEOD-69733 | biostudies-arrayexpress
TranscriptomicsTHOC5, a member of the mRNA export complex, is essential for hematopoiesis in vivo and is required for CSF-1 induced macrophage differentiation
THOC5, a member of the mRNA export complex, is essential for hematopoiesis in vivo and is required for CSF-1 induced macrophage differentiation, thereby playing a key role in the mRNA export of immediate early genes induced by CSF-1 stimulation. Hematopoiesis, growth, differentiation, and commitment...
ORGANISM(S): Mus musculus 
2013-12-24 | E-GEOD-41170 | biostudies-arrayexpress
TranscriptomicsRNAi knock down of export factors in Drosphila Schneider cell lines
Six different endogenous Drosophila proteins (all potential export factors) have been inactivated using RNAi and leptomycin B (LMB) treatments. Five different endogenous Drosophila proteins (all potential export factors) have been targeted by RNAi. The effect on mRNA export is analyzed by monitoring...
ORGANISM(S): Drosophila melanogaster 
2003-05-15 | E-MEXP-4 | biostudies-arrayexpress
TranscriptomicsSelective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP
The nuclear phase of the gene expression pathway culminates in the export of mature mRNAs to the cytoplasm through nuclear pore complexes (NPCs). GANP (Germinal-centre Associated Nuclear Protein) promotes the transfer to NPCs of mRNAs bound to the transport factor NXF1. Here, we demonstrate that GAN...
ORGANISM(S): Homo sapiens 
2014-02-14 | E-GEOD-54481 | biostudies-arrayexpress
TranscriptomicsHOS1 versus hos1 mRNA sequencing
Sequencing of mRNA extracted from 14 day old Columbia and hos1-3 seedlings grown at 22C 12:12 light dark Cold acclimation has been shown to be attenuated by the degradation of the INDUCER OF CBF EXPRESSION1 protein by the E3 ubiquitin ligase HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1 (HOS1). H...
ORGANISM(S): Arabidopsis thaliana 
2016-07-21 | E-GEOD-84606 | biostudies-arrayexpress
TranscriptomicsHuman inositol polyphosphate multikinase regulates transcript-selective nuclear mRNA export to preserve genome integrity
Messenger (m)RNA export from the nucleus is essential for eukaryotic gene expression. Here, we identify a transcript-selective nuclear export mechanism affecting certain human transcripts, enriched for functions in genome duplication and repair, controlled by inositol polyphosphate multikinase (IPMK...
ORGANISM(S): Homo sapiens 
2013-09-27 | E-GEOD-49658 | biostudies-arrayexpress
TranscriptomicsThe THO complex regulates pluripotency gene mRNA export to control embryonic stem cell self-renewal and somatic cell reprogramming (microarray)
Embryonic stem cell (ESC) self-renewal and differentiation are governed by a broad-ranging regulatory network. Although the transcriptional regulatory mechanisms involved have been investigated extensively, post-transcriptional regulation is still poorly understood. Here we describe a critical role ...
ORGANISM(S): Mus musculus 
2013-11-30 | E-GEOD-51496 | biostudies-arrayexpress
TranscriptomicsThe THO complex regulates pluripotency gene mRNA export to control embryonic stem cell self-renewal and somatic cell reprogramming (RNA-IP)
Embryonic stem cell (ESC) self-renewal and differentiation is governed by a comprehensive regulatory network. Although the transcriptional regulation has been extensively investigated, post-transcriptional mechanisms controlling the ESC state are poorly understood. Here we describe a critical role o...
ORGANISM(S): Mus musculus 
2013-11-30 | E-GEOD-48436 | biostudies-arrayexpress
ProteomicsA heterochromatin specific RNA export pathway facilitates piRNA production
PIWI-interacting RNAs (piRNAs), a class of small RNAs that guide transposon silencing in animals, are processed in the cytoplasm from RNA Polymerase II transcripts. How piRNA precursors, which often lack RNA maturation signatures and thereby violate quality control checkpoints, achieve nuclear expor...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-03-29 | PXD012717 | Pride
Export Drosophila Ovary Pirna 
ProteomicsALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export
Mutations in Matrin 3 have recently been linked to ALS, though the mechanism of disease in these patients is still unknown. To define the protein interactome of wild-type and ALS-linked MATR3 mutations, we performed immunoprecipitation followed by mass spectrometry experiments in NSC-34 cells expres...
ORGANISM(S): Mus musculus (Mouse) 
2017-11-15 | PXD007710 | Pride
Als Lc/ms-ms Trex Matrin-3 
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