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ProteomicsConfetti: A Multi-protease Map of the HeLa Proteome for Comprehensive Proteomics
Tryptic digestion proteomics gives limited coverage of many protein sequences as many proteins produce tryptic peptides that cannot be observed easily. Confetti is our comprehensive map of coverage for the HeLa proteome, using 48 different digests and CID/HCD LC-MS/MS analyses. An associated dataset...
ORGANISM(S): Homo sapiens (Human) 
2014-04-16 | PXD000900 | Pride
Lc-ms/ms Digestion Enzymes Non-tryptic 
ProteomicsMulti-protease approach for the improved identification and molecular characterization of small proteins and short open reading frame-encoded peptides
The identification of proteins below 70 amino acids in bottom-up proteomics is still a challenging task due to the limited number of peptides generated by proteolytic digestion. This includes the short open reading frame-encoded peptides (SEP), which are a subset of the small proteins that were not ...
ORGANISM(S): Methanosarcina mazei Go1 
2021-04-01 | PXD023921 | Pride
Lc-msms Peptidomics Sorf Sep 
ProteomicsCasein phoshopeptides identification by partial-, double-dephosphorylation and endoGluC digestion
The aim of the present study was the development of a relatively simple method without enrichment step to detect, in a mixture of peptides and phosphopeptides, a maximum of CPPs, including multi-phosphorylated and large-size CPPs, by using high pressure liquid chromatography (HPLC), high-resolution ...
ORGANISM(S): Bos taurus (Bovine) 
2021-09-29 | PXD027132 | Pride
Dephosphorylation Mass spectrometry Casein phosphopeptides Endogluc digestion 
UnknownSARS-CoV-2 spike protein-, main protease- and papain-like-protease-targeting peptides from seed proteins following gastrointestinal digestion: An in silico study.
Not available
S-EPMC7832997 | biostudies-literature
ProteomicsProteome-wide Analysis of Palmitoylated Proteins in Macrophages Reveals Novel Insights into Early Immune Signaling
To understand protein S-palmitoylation's role in macrophage reprogramming, dynamic changes were comprehensively mapped in immortalized mouse macrophages during early LPS stimulation using acyl-biotin exchange (ABE) proteomics enhanced with multi-protease digestion. This approach identified 2613 puta...
ORGANISM(S): Mus musculus (Mouse) 
2026-08-20 | PXD063463 | Pride
Macrophage Acyl-biotin exchange (abe) Palmitoylation Immune signaling S-acylation Post-translational modification Lipopolysaccharide (lps) Multi-protease digestion Proteomics 
OtherConfetti: A Multi-protease Map of the HeLa Proteome for Comprehensive Proteomics
Data from ProteomeXchange, PXD ID: PXD000900. Data file: HeLa_ArgC-Elastase_CID_2.mgf. Published as part of Mol Cell Proteomics. 2014 Apr 2 . From the Abstract: {{i}} ... In an attempt to improve protein sequence coverage and to target regions of proteins that do not generate useful tryptic peptide...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM11210026998 | GPMDB
Resource Reanalysis 
UnknownInter-laboratory study of an optimised peptide mapping workflow using automated trypsin digestion for monitoring monoclonal antibody product quality attributes.
Not available
S-EPMC7496030 | biostudies-literature
ProteomicsCBB staining showed beneficial effects on proteomic analysis employing PAGE separation followed with whole-gel slicing, in-gel digestion and quantitative LC-MS/MS
Proteomic analysis by combining PAGE separation, gel slicing and slice-by-slice LC-MS/MS has been frequently reported in recent years. Since the MS analysis would provide identities and quantities of the proteins along the whole lane, visualization by dye staining could be skipped to save time and ...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-02-16 | PXD011689 | Pride
Sds-page In-gel digestion Proteomic analysis Cbb r-250 staining Whole gel slicing Quantitative lc-ms/ms Nondenaturing page 
UnknownRhipicephalus microplus and Ixodes ovatus cystatins in tick blood digestion and evasion of host immune response.
Not available
S-EPMC4340882 | biostudies-literature
ProteomicsCoMPaseD - Protease Score Prediction for the Analysis of Small Proteins
In proteome studies, the application of alternative proteases, exclusively or in addition to trypsin, often increases protein sequence or proteome coverage. It has recently been shown that, in particular, the analysis of small proteins benefits from such multi-protease approaches. In the accompanyin...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2026-01-23 | PXD062213 | Pride
Bacillus subtilis Orbitrap elite Alternative proteases Gluc Lysarginase 
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