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TranscriptomicsGlobal Run-On sequencing (GRO-seq) of HeLa cells under CDK9 inhibitor tratment to study elongation checkpoints at both ends of RNA polymerase II-transcribed genes.
Global Run-On sequencing (GRO-seq) was carried out on nuclei isolated from HeLa cells after 30 minutes of treatment with CDK9 inhibitors KM05382 and DRB and from two untreated controls. Libraries of nascent RNAs were generated and sequenced using Illumina Hi-seq technology. GRO-seq reads were proces...
ORGANISM(S): Homo sapiens 
2015-02-26 | E-MTAB-3360 | biostudies-arrayexpress
ProteomicsCharacterization of the SF3B1 interactome
CDK9 is a critical kinase required for the productive transcription of protein-coding genes by RNA polymerase II (pol II). As part of P-TEFb, CDK9 phosphorylates the carboxyl-terminal domain (CTD) of pol II and elongation factors, including SPT5, which allows pol II to elongate past the early elonga...
ORGANISM(S): Homo sapiens (Human) 
2022-08-22 | PXD033694 | Pride
Transcription Splicing Sf3b1 Interactome 
ProteomicsCAPTURE of the human U2 snRNA genes
In order to identify factors involved in transcription of human snRNA genes and 3’ end processing of the transcripts, we have carried out CRISPR affinity purification in situ of regulatory elements (CAPTURE), which is deadCas9-mediated pull-down, of the tandemly-repeated U2 snRNA genes in human cell...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD033580 | Pride
Transcription Snrna Interactome 
ProteomicsIdentification of CDK9 targets using DRB and quantitative phosphoproteomics
CDK9 is a critical kinase required for the productive transcription of protein-coding genes by RNA polymerase II (pol II) in higher eukaryotes. Phosphorylation of targets including the elongation factor SPT5 and the carboxyl-terminal domain (CTD) of RNA pol II allow the polymerase to pass an early e...
ORGANISM(S): Homo sapiens (Human) 
2022-08-22 | PXD026720 | Pride
Hela Silac Drb Phosphoproteomics Cdk9 
TranscriptomicsAnalysis of transcriptome changes in HeLa cells after knock-down of variant U1.8 snRNA
U1 small nuclear (sn)RNA, required for splicing of pre-mRNA, is encoded by genes on chromosome 1p36. Imperfect copies of these ‘true’ (t)U1 snRNA genes, located on chromosome 1q12-21, were thought to be pseudogenes. However, many of these ‘variant’ (v)U1 snRNA genes produce fully-processed transcrip...
ORGANISM(S): Homo sapiens 
2012-10-01 | E-GEOD-37623 | biostudies-arrayexpress
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