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TranscriptomicsMultiple roles for LEC in initiation and elongation phases of snRNA gene transcription
The small nuclear RNA (snRNA) genes have been widely used as a model system for understanding transcriptional regulation due to unique aspects of their promoter structure, selectivity for either RNA Polymerase (Pol) II or III and a unique mechanism of termination that is tightly linked with the prom...
ORGANISM(S): Homo sapiens 
2013-08-08 | E-GEOD-47938 | biostudies-arrayexpress
TranscriptomicssnRNA-seq of whole hearts from adult BL6 mice
We used snRNA-seq to investigate an entire adult mammalian heart of BL6 mice. Whole hearts were harvested from 4 male mice (12 weeks) after cervical dislocation. The hearts were pooled and nuclei isolated using the Nuclei PURE Prep isolation kit (Sigma-Aldrich, Darmstadt, Germany) according to the m...
ORGANISM(S): Mus musculus 
2020-03-16 | E-MTAB-8848 | biostudies-arrayexpress
TranscriptomicsSHAPE-Seq of human U2 snRNA and U4 pre-snRNA
Spliceosomal snRNA are key components of small nuclear ribonucleoprotein particles (snRNPs), the building blocks of the spliceosome. The biogenesis of snRNPs is a complex process involving multiple cellular and subcellular compartments, the details of which are yet to be described. In short, the snR...
ORGANISM(S): Homo sapiens 
2023-09-08 | E-MTAB-13248 | biostudies-arrayexpress
TranscriptomicssnRNA-seq of whole hearts from adult fzt:DU mice
We used snRNA-seq to investigate for the first time an entire adult mammalian heart. To avoid potential aberrations due to inbreeding, we relied on an outbred mice strain (Fzt:DU) (Dietl, G.; Langhammer, M.; Renne, U. Model simulations for genetic random drift in the outbred strain Fzt:DU. Arch. Ani...
ORGANISM(S): Mus musculus 
2020-02-22 | E-MTAB-8751 | biostudies-arrayexpress
ProteomicsCAPTURE of the human U2 snRNA genes
In order to identify factors involved in transcription of human snRNA genes and 3’ end processing of the transcripts, we have carried out CRISPR affinity purification in situ of regulatory elements (CAPTURE), which is deadCas9-mediated pull-down, of the tandemly-repeated U2 snRNA genes in human cell...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD033580 | Pride
Transcription Snrna Interactome 
ProteomicsStructural basis of SNAPc-dependent snRNA transcription intiation by RNA polymerase II
RNA Polymerase II (Pol II) carries out transcription of both protein-coding and non-coding genes. Whereas Pol II initiation at protein-coding genes has been studied in detail, Pol II initiation at non-coding genes such as small nuclear RNA (snRNA) genes is not understood at the structural level. Her...
ORGANISM(S): Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2023-03-11 | PXD033638 | Pride
Snrna Rna polymerase ii 
TranscriptomicsThe little elongation complex (LEC) regulates small nuclear RNA transcription
The Eleven-nineteen Lysine-rich Leukemia (ELL)-containing Super Elongation Complex (SEC) containing P-TEFb is a key regulator in the expression of HOX genes in Mixed Lineage Leukemia (MLL)-based leukemia. We have identified an SEC-like complex in Drosophila, as well as a distinct ELL-containing comp...
ORGANISM(S): Drosophila melanogaster 
2011-12-22 | E-GEOD-32120 | biostudies-arrayexpress
ProteomicsIdentification of U1 snRNA associated proteins
In this project, we isolate U1 snRNA associated proteins in Arabidopsis thaliana. We used an antisense oligonucleotide specific for the U1 snRNA and analyzed associated proteins by mass spectrometry. As a control, the same experiments were performed with U2 snRNA- and lacZ-specifc antisense oligonuc...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-12-04 | PXD045484 | Pride
U1 snrna Arabidopsis U2 snrna Comprehensive isolation by rna binding proteins (chirp) 
TranscriptomicssnRNA-seq for Macaque LIP
snRNA-sequencing data for macaque LIP area. We isolated single nuclei from the LIP region. annotating the dataset with the canonical classification and integration with other datasets.
ORGANISM(S): Macaca fascicularis 
2026-05-31 | E-MTAB-13364 | biostudies-arrayexpress
TranscriptomicsAnalysis of transcriptome changes in HeLa cells after knock-down of variant U1.8 snRNA
U1 small nuclear (sn)RNA, required for splicing of pre-mRNA, is encoded by genes on chromosome 1p36. Imperfect copies of these ‘true’ (t)U1 snRNA genes, located on chromosome 1q12-21, were thought to be pseudogenes. However, many of these ‘variant’ (v)U1 snRNA genes produce fully-processed transcrip...
ORGANISM(S): Homo sapiens 
2012-10-01 | E-GEOD-37623 | biostudies-arrayexpress
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