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ProteomicsProximity-dependent biotinylation detects association between stress granule associated proteins and SARS coronavirus nonstructural protein 1
Severe acute respiratory syndrome coronavirus (SARS-CoV and SARS-CoV-2) nonstructural protein 1 (nsp1) is a critical viral protein that suppresses host gene expression by blocking the assembly of the ribosome on host messenger RNAs (mRNA). Using proximity-dependent biotinylation assay followed by pr...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD025980 | Pride
Nsp1 Stress granule Sars-cov Sars-cov-2 Bioid2 
TranscriptomicsTranscription profiling of human breast stroma and epithelium after curative radiotherapy to investigate the pathogenesis of late radiation injury in humans
Gene expression profiling of normal tissues after curative radiotherapy was carried out to investigate the pathogenesis of late radiation injury in humans. Irradiated and control normal breast tissue was collected from patients undergoing bilateral mastectomy for ipsilateral tumour relapse or proph...
ORGANISM(S): Homo sapiens 
2008-08-12 | E-TABM-419 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of normal and malignant human breast epithelial cells
Multiple genome-wide microarrays have been used to analyse the transcriptomes of immunomagnetically separated normal human luminal epithelial and myoepithelial cells, as well as primary malignant breast epithelial cells.
ORGANISM(S): Homo sapiens 
2006-09-29 | E-TABM-66 | biostudies-arrayexpress
TranscriptomicsRNA-seq analysis of K562 cells (K562-shX) with stable integration of a tet-inducible shRNA targeting a candidate (X) transcription factor from ENCODE/HudsonAlpha-MyersLab
K562-shX cells are made in an effort to validate TFBS data and ChIP-seq antibodies in Myers lab (GSE32465). K562 cells are transduced with lentiviral vector having Tet-inducible shRNA targeting a transcription factor gene. Cells with stable integration of shRNA constructs are selected using puromyci...
ORGANISM(S): Homo sapiens 
2011-12-01 | E-GEOD-33816 | biostudies-arrayexpress
TranscriptomicsTranscription profiling, comparative genomic hybridization, genotyping and methylation profiling by array of human breast cancer cell lines
Integrative analysis of breast cancer cell lines, using expression, comparative genomics, SNP and methylation profiling
ORGANISM(S): Homo sapiens 
2012-11-09 | E-TABM-928 | biostudies-arrayexpress
TranscriptomicsSystems toxicology assessment of a representative e-liquid formulation using human primary bronchial epithelial cells
The development of reduced-risk products is aimed at providing adult smokers, who are unable to quit, with alternatives to cigarettes that present less risk of harm and smoking-related disease. The use of flavors in the development of these reduced-risk products fulfils an important role in product ...
ORGANISM(S): Homo sapiens 
2019-11-01 | E-MTAB-7748 | biostudies-arrayexpress
TranscriptomicsComparative genomic hybridization of human breast cancer cell lines
AM_BreastCancerCellLineExpressionaCGH
ORGANISM(S): Homo sapiens 
2009-02-03 | E-TABM-593 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse mammary cell subpopulations isolated by flow cytometry, on the basis of staining with antibodies against CD45, CD49f, Sca-1 and the 33A10 antigen
We have carried out microarray-based gene expression analysis of cell subpopulations isolated by flow cytometry, on the basis of staining with antibodies against CD45, CD49f, Sca-1 and the 33A10 antigen, from freshly prepared mouse mammary glands. RNA isolated from cell populations was co-hybridised...
ORGANISM(S): Mus musculus 
2007-11-20 | E-MEXP-423 | biostudies-arrayexpress
ProteomicsMultiple Reaction Monitoring assays targeting modified peptides from Plasmodium histones at 0, 18 and 36 hours post-invasion
Acid-extracted histones from the 0-hpi_2, 18-hpi_1 and 36-hpi_2 samples previously analyzed for PTMs by MudPIT were digested with endoproteinase Arg-C (RC) or endoproteinase Lys-C followed by Glu-C (KCEC). Digested histone peptides were separated on a 15cm nano-reverse phase (RP) column using a 4-hr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2016-06-02 | MSV000079792 | MassIVE
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