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ProteomicsHuman Cardiac Fibroblast BioID2 Interactome for SKI and TAZ(WWTR1)
We used adenoviral-mediated overexpression of MYC-BioID2, MYC-BioID2-SKI, MYC-BioID2-WWTR1 (TAZ) in human primary cardiac fibroblasts to elucidate the interaction between SKI and the Hippo signaling pathway. Original data is also available on the Global Proteome Machine (http://hs2.proteome.ca/tande...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD018246 | Pride
Taz Bioid2 Cardiac fibroblast Heart Hippo Ski 
ProteomicsUsing BioID2 to map domain specific Ku70 protein interactors
To determine if the proximity-dependent biotin identification (BioID2) technique could identify and map domain and region specific protein interactors, we conducted BioID2 with various segments of the Ku70 protein in the HEK293 cell line and compared the different datasets. To our knowledge, this is...
ORGANISM(S): Homo sapiens (Human) 
2021-04-22 | PXD020165 | Pride
Ku70 Bioid2 Hek293 Lc-esi-ms/ms 
ProteomicsGPI-Anchored Ligand-BioID2-Tagging System Identifies proteins Mediating Zika Virus Entry
Identification of host factors facilitating pathogen entry is critical for preventing infectious diseases. Here, we report a tagging system consisting of a viral receptor-binding protein (RBP) linked to BioID2, which is expressed on the cell surface via a GPI anchor. Using VSV or Zika virus (ZIKV) R...
ORGANISM(S): Homo Sapiens 
2022-10-12 | PXD037307 |
ProteomicsIdentification of proximal interactome of BioID2-tagged mitochondrial Top3α
To interrogate the role of Top3a in mtDNA replication progression, we studied the interaction of mitochondrial Top3a with known mitochondrial DNA maintenance proteins using proximity labelling proteomics. For this experiment, FlpIN-TRex 293 cell line expressing mitochondrial Top3a tagged with BioID2...
ORGANISM(S): Homo sapiens (Human) 
2022-07-22 | PXD035416 | Pride
Human Q exactive Mitochondria Bioid2 Top3α 
ProteomicsIdentifying global and mtCU independent MICU1 interactome utilizing BioID2 based proximity labeling and screening
MICU1 is a Ca2+-binding protein that regulates the mitochondrial Ca2+ uniporter channel (mtCU ) and mitochondrial Ca2+ (mCa2+) uptake. MICU1 knockout mice display perinatal lethality and disorganized mitochondrial architecture. These phenotypes are distinct from other mtCU loss-of-function models an...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD028462 | Pride
Bioid2 Mitochondria Calcium Proximity-labelling Mcu Micu1 
ProteomicsBioID2(Gly40Ser)-SEC22A proximity biotinylation in HeLa cells
Proximity labelling using transient expression of the same exogenous BioID2(Gly40Ser)-SEC22A construct in wild-type HeLa cells and in otherwise isogenic RAB3GAP1-, RAB3GAP2- and RAB18-null HeLa cell lines.
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD016404 | Pride
Human Rab3gap2 Sec22a Hela Rab18 Rab3gap1 Bioid Bioid2 
UnknownIdentification of Ku70 Domain-Specific Interactors Using BioID2.
Not available
S-EPMC8001828 | biostudies-literature
UnknownScreening of novel Midkine binding protein by BioID2-based proximity labeling.
Not available
S-EPMC8438011 | biostudies-literature
UnknownGPI-anchored ligand-BioID2-tagging system identifies Galectin-1 mediating Zika virus entry.
Not available
S-EPMC9668739 | biostudies-literature
ProteomicsComprehensive analysis of the host-virus interactome of SARS-CoV-2
Host-virus protein-protein interaction is the key component of SARS-CoV-2 life cycle, which can be targeted for therapeutic intervention. Thus, we conducted a comprehensive interactome study between the virus and host cell using tandem affinity purification (TAP) and BioID2 labeling strategies. In a...
ORGANISM(S): Homo sapiens (Human) 
2021-06-29 | PXD023209 | Pride
Sfb-tap Sars-cov-2 Human coronavirus Host-virus protein-protein interaction Bioid2 Interactome 
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