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ProteomicsGeneration and Characterization of a Conditional Ku70 Knockout Cell Line
In this study we characterize and investigate the effect of the loss of Ku70 protein in a conditional Ku70 knockout TREx-293 cell system. Loss of Ku70 protein levels was directly correlated with a loss of cell viability, supporting the observation that Ku performs an essential role in human cells. I...
ORGANISM(S): Homo sapiens (Human) 
2023-07-27 | PXD036297 | Pride
Ku70 hek293 
ProteomicsHuman Ku70 WT AP-MS - Mapping the Ku interactome using proximity-dependent biotin identification in human cells
Affinity purification coupled to mass spectrometry is often used as a complementary technique to BioID experiments, and the data presented here complements another project. To infer processes involving Ku70, we used AP-MS to identify candidate proteins that may interact with Ku70 and compared these ...
ORGANISM(S): Homo sapiens (Human) 
2019-01-03 | PXD010930 | Pride
Ku70 Ap-ms Hek293 
ProteomicsHEK293 Ku70 WT BioID - Mapping the Ku interactome using proximity-dependent biotin identification in human cells
We conducted proximity-dependent biotin identification (BioID) using wild-type Ku70 in HEK293 cells. Ku70 is part of a protein complex known as Ku, which is involved in a variety of functions throughout the cell. By identifying candidate protein interactors, we can infer new cellular processes that ...
ORGANISM(S): Homo sapiens (Human) 
2019-01-03 | PXD010931 | Pride
Ku70 Bioid Hek293 
ProteomicsScreening for Ku70 phosphoS155-dependent proximal proteins
To identify Ku70 phosphoS155-specific proximal protein interactors, we used the proximity-dependent biotin identification (BioID2) technique with wild-type Ku70, Ku70 S155A, and Ku70 S155D in the doxycycline-inducible T-REx 293 cell line and compared the different datasets.
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD025581 | Pride
Ku70 S155 T-rex 293 Bioid2 Lc-esi-ms/ms 
TranscriptomicsTranscriptome of Yarrowia lipolytica strain E150 delta-Ku70 on glucose
batch culture of Yarrowia lipolytica strain E150 delta-Ku70
ORGANISM(S): Yarrowia lipolytica 
2012-12-31 | E-MTAB-940 | biostudies-arrayexpress
ProteomicsUsing BioID2 to map domain specific Ku70 protein interactors
To determine if the proximity-dependent biotin identification (BioID2) technique could identify and map domain and region specific protein interactors, we conducted BioID2 with various segments of the Ku70 protein in the HEK293 cell line and compared the different datasets. To our knowledge, this is...
ORGANISM(S): Homo sapiens (Human) 
2021-04-22 | PXD020165 | Pride
Ku70 Bioid2 Hek293 Lc-esi-ms/ms 
ProteomicsHMGB1 signaling phosphorylates Ku70 and impairs DNA damage repair in Alzheimer’s disease pathology
DNA damage is increased in Alzheimer’s disease (AD), while the underlying mechanisms are unknown. Here, we employ comprehensive phosphoproteome analysis, and identify abnormal phosphorylation of 70 kDa subunit of Ku antigen (Ku70) at Ser77/78, which prevents Ku70-DNA interaction, in human AD postmor...
ORGANISM(S): Homo sapiens (Human) 
2022-02-15 | PXD028089 | Pride
Hmgb1 Ku70 Phosphorylation Alzheimer's disease 
GenomicsAspergillus niger strain:delta ku70
Aspergillus niger delta ku70 gene expression profiling - 1.2.8.1 transcriptome
PRJNA1299590 | ENA
GenomicsAspergillus niger strain:delta ku70
Aspergillus niger delta ku70 gene expression profiling - 1.2.8.2 transcriptome
PRJNA1299561 | ENA
GenomicsAspergillus niger strain:delta ku70
Aspergillus niger delta ku70 gene expression profiling - 1.3.2.2 transcriptome
PRJNA1299562 | ENA
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