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Bone marrow-derived macrophages from mice were treated with recombinant Ssa1, a protein enriched in the hypoxic secretome of Candida albicans.
ORGANISM(S): Mus Musculus (mouse) 
Human lung epithelial A549 cells were exposed to radon 1,000 Bq/m3 for 30 min, repeated daily for seven days. The control cells were cultured under the same conditions but without radon exposure. Cells were washed three times with ice-cold 1x phosphate buffer saline supplemented with protease inhibi...
ORGANISM(S): Homo Sapiens (human) 
Candida albicans were cultured under normal and hypoxic conditions and collected secretome from the culture supernatant with centrifugation. The secretome contains secretory proteins, extracellular vehicles, and others.
ORGANISM(S): Candida Albicans 
The proteomic analysis of liver samples from mice administered proteoglycan (PG) for 9 days or untreated control mice. Liver samples from mice administered PG for 9 days or untreated control mice (n = 6 per group) were homogenized in PBS. The proteins were precipitated using acetone. The supernatant...
ORGANISM(S): Mus Musculus (mouse) 
Helicobacter pylori strain TN2wt was suspended in Brucella broth supplemented with 5% depleted exosomes FCS. The initial optical density at 600 nm of the bacterial suspension was adjusted to 0.02 in 200 mL of Brucella broth + 0.5% depleted exosome FCS. H. pylori was grown in a 500 mL flask under mic...
ORGANISM(S): Helicobacter Pylori 
Helicobacter pylori strain ATCC51932 (Tx30a) was suspended in Brucella broth supplemented with 5% depleted exosomes FCS. The initial optical density at 600 nm of the bacterial suspension was adjusted to 0.02 in 200 mL of Brucella broth + 0.5% depleted exosome FCS. H. pylori was grown in a 500 mL fla...
ORGANISM(S): Helicobacter Pylori 
Human gastric adenocarcinoma AGS cells were infected with H. pylori Tx30a or TN2wt at a multiplicity of infection = 100 and incubated at 5% CO2,37oC for 48 h. The cells/debris were removed from the cell culture supernatant by centrifugation at 2,000 x g for 30 min at 4oC and filtered through a 0.45 ...
ORGANISM(S): Homo Sapiens (human) 
Acinetobacter baumannii were cultured in Brain Heart Infusion medium (BD Bioscience). For normoxic condition, the cultures were shaken at 125 rpm for 24 h, whereas for hypoxic condition, the cultures were placed under static condition for 48 h. Outer membrane vesicles from A. baumannii (AbOMVs) in t...
ORGANISM(S): Acinetobacter Baumannii 
Staphylococcus aureus strain ATCC 1718 was inoculated at 0.5% in 400 mL of Brain Heart Infusion broth for 8 h under aerobic conditions. The supernatant was collected by centrifugation twice at 5,000 x g, 4°C for 20 min, filtrated through a 0.45 µm filter to remove all remaining bacterial cells. S. a...
ORGANISM(S): Staphylococcus Aureus 
PaOMVs in the culture supernatant of P. aeruginosa were harvested by ultracentrifugation at 100,000 × g, 4℃ for 90 min using a Himac CP80WX Preparative Ultracentrifuge (HITACHI, Tokyo, Japan). After removal of the supernatant, the pellet was washed twice and suspended in ice-cold sterile phosphate-b...
ORGANISM(S): Pseudomonas Aeruginosa 
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