We optimized a protocol to analyze the placental proteome by means of label-free nano-LC-MS/MS mass spectrometry with regard to sample treatment, protein extraction, and protein digestion, in order to obtain a high protein concentration for identification of a specific protein signature according to...
Shotgun proteomics of normal versus germ-free mouse terminal ileum. 39 mice. Male and female. BALBc and C57BL10. The samples were labeled using iTRAQ 8plex and analyzed using a nano-LC-LTQ-Orbitrap-Velos-MSMS. This is the proteomics data from an n-omics project.
A blue-light receptor phototropin (phot) mediates chloroplast relocation responses. Upon receiving blue light, phot undergoes autophosphorylation via the serine/threonine kinase domain. The autophosphorylation levels of phot change in response to different blue-light intensities and temperature, and...
Primary human umbilical vein endothelial cells (HUVECs, n=4) were exposed to low-magnitude pre-conditioning FSS (1.5 dynes/cm2) 18h using the Ibidi pump system followed by exposure to the arterial- (15 dynes/cm2) or venous-magnitude (1.5 dynes/cm2) FSS for 24h. Conditioned media was collected to ana...
Nano LC-MS/MS was carried out, which A549 cell lysates were immunoprecipitated with mouse anti-kindlin-2 antibody (clone 3A3.538) or control mouse IgG (Santa Cruz, Cat# sc-2025).
Identification by nano-LC-MS/MS of NT5DC2 as a protein binding to tyrosine hydroxylase: Down-regulation of NT5DC2 by siRNA increases catecholamine synthesis in PC12D cells
Bacterial extracellular vesicles (BEVs) produced by members of the intestinal microbiota can contribute not only to digestion but also mediate microbe-host cell communication via the transfer of functional biomolecules to mammalian host cells. An unresolved question is what host factors and conditio...
DNA, RNA and protein were extracted from the culture and subjected to massive parallel sequencing and nano-LC-MS-MS respectively Combination of these methods enabled the reconstruction of the complete genome sequence of M oxyfera from the metagenome and identification of the functionally relevant en...
