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GenomicsBiological chromodynamics: a general method for measuring protein occupancy across the genome by calibrating ChIP-seq
Sequencing DNA fragments associated with proteins following in vivo cross-linking with formaldehyde (known as ChIP-seq) has been used extensively to describe the distribution of proteins across genomes. It is not widely appreciated that this method merely estimates a protein’s distribution and canno...
ORGANISM(S): Saccharomyces cerevisiae 
2015-07-10 | E-GEOD-69907 | biostudies-arrayexpress
TranscriptomicsSpo13 facilitates monopolin recruitment to kinetochores and regulates maintenance of centromeric cohesion
BACKGROUND: Cells undergoing meiosis perform two consecutive divisions after a single round of DNA replication. During the first meiotic division, homologous chromosomes segregate to opposite poles. This is achieved by (1) the pairing of maternal and paternal chromosomes via recombination producing ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-11 | E-GEOD-4813 | biostudies-arrayexpress
TranscriptomicsEvidence that Loading of Cohesin Onto Chromosomes Involves Opening of Its SMC Hinge
Cohesin is a multisubunit complex that mediates sister-chromatid cohesion. Its Smc1 and Smc3 subunits possess ABC-like ATPases at one end of 50 nm long coiled coils. At the other ends are pseudosymmetrical hinge domains that interact to create V-shaped Smc1/Smc3 heterodimers. N- and C-terminal domai...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-09 | E-GEOD-4827 | biostudies-arrayexpress
TranscriptomicsMonopolar attachment of sister kinetochores at meiosis I requires casein kinase 1.
The segregation of maternal centromeres away from the paternal ones during the first division of meiosis depends on the attachment of sister kinetochores to microtubules emanating from the same spindle pole. In budding yeast monopolar attachment requires the recruitment to kinetochores of a protein ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-10 | E-GEOD-4792 | biostudies-arrayexpress
TranscriptomicsProtein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I
Segregation of homologous maternal and paternal centromeres to opposite poles during meiosis I depends on post-replicative crossing over between homologous non-sister chromatids, which creates chiasmata and therefore bivalent chromosomes. Destruction of sister chromatid cohesion along chromosome arm...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-11 | E-GEOD-4791 | biostudies-arrayexpress
TranscriptomicsDifferential gene expression profiling in the presence and absence of cohesin in Drosophila salivary glands (gene expression data)
Analysis of differential gene expression in third instar Drosophila salivary glands in the absence versus presence of cohesin. ABSTRACT: Developmental abnormalities observed in Cornelia de Lange Syndrome (CdLS) have been genetically linked to mutations in the cohesin machinery. These findings raise ...
ORGANISM(S): Drosophila melanogaster 
2010-12-22 | E-GEOD-21844 | biostudies-arrayexpress
TranscriptomicsA direct role for cohesin in genome-wide gene regulation and Ecdysone-response in Drosophila salivary gland
Developmental abnormalities observed in Cornelia de Lange Syndrome (CdLS) have been genetically linked to mutations in the cohesin machinery. These findings raise the possibility that cohesin, in addition to its canonical function of mediating sister chromatid cohesion, might also be involved in reg...
ORGANISM(S): Drosophila melanogaster 
2011-03-22 | E-GEOD-21874 | biostudies-arrayexpress
TranscriptomicsPcs1 and Mde4 are inner centromere proteins required to prevent merotelic kinetochore orientation
Chromosome segregation depends on proper attachment of sister kinetochores to microtubules. Merotelic kinetochore orientation is an error which occurs when a single kinetochore is attached to microtubules emanating form opposite poles. Mechanisms preventing or correcting the merotelic attachment mus...
ORGANISM(S): Schizosaccharomyces pombe 
2010-06-10 | E-GEOD-5257 | biostudies-arrayexpress
GenomicsReleasing activity disengages cohesin’s Smc3/Scc1 interface in a process blocked by acetylation
Sister chromatid cohesion conferred by entrapment of sister DNAs within a tripartite ring formed between cohesin’s Scc1, Smc1, and Smc3 subunits is generated during S and eventually destroyed at anaphase through cleavage of Scc1 by separase. Throughout the cell cycle, cohesin’s association with ...
ORGANISM(S): Saccharomyces cerevisiae 
2016-02-18 | E-GEOD-76890 | biostudies-arrayexpress
GenomicsCohesin releases DNA through asymmetric ATPase-driven ring opening
Cohesin stably holds together the sister chromatids from S phase until mitosis. To do so, cohesin must be protected against its cellular antagonist Wapl. Eco1 acetylates cohesin’s Smc3 subunit, which locks together the sister DNAs. We used yeast genetics to dissect how Wapl drives cohesin from chr...
ORGANISM(S): Saccharomyces cerevisiae 
2016-02-18 | E-GEOD-76891 | biostudies-arrayexpress
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